Nong Yvonne, Walsh Dana M, Maloh Jessica, Dadlani Manoj, Sivamani Raja
SUNY Downstate Medical Center, Brooklyn, NY, USA.
Integrative Skin Science and Research, Sacramento, CA, USA.
J Psoriasis Psoriatic Arthritis. 2024 Jul;9(3):98-107. doi: 10.1177/24755303241242357. Epub 2024 May 7.
Psoriasis is an immune-mediated cutaneous disease that may have shifts in the skin microbiome. Prior research on the skin microbiome in psoriasis has been limited to rRNA based approaches that lack resolution of taxonomic and functional level assessment.
To further illuminate strain and sub-strain level analysis of psoriatic lesions using the CosmosID-HUB Microbiome pipeline.
A previous study completed by Tett et al recruited patients with psoriasis who had skin microbiome samples taken from psoriatic plaques on the ear and the elbow as well as sites on the skin unaffected by psoriasis. They performed whole genome shotgun sequencing and made their dataset publicly available. We analyzed the dataset using the CosmosID-HUB Microbiome pipeline to evaluate the strain and sub-strain taxonomic analysis as well as functional gene profiling.
When analyzed with the CosmosID pipeline, both ear and elbow sites in affected areas had decreased alpha diversity compared to unaffected areas. There was an increased relative abundance of and Corynebacteria at affected sites. We identified distinguishing species and strains of the yeast Malassezia, including . that were significantly enriched in healthy elbow samples. Vitamin B12 production genes were not present in psoriatic skin whereas it was present in healthy samples, supporting the notion of relative vitamin B12 deficiency in psoriatic plaques. Phage analysis revealed a greater diversity of Staphylococcus-related phages in unaffected elbow samples.
A greater diversity of microbial strains and their functional roles identified in this study may help to tailor treatment for psoriasis.
银屑病是一种免疫介导的皮肤病，其皮肤微生物群可能会发生变化。先前关于银屑病皮肤微生物群的研究仅限于基于rRNA的方法，这些方法缺乏分类学和功能水平评估的分辨率。
使用CosmosID-HUB微生物组分析流程进一步阐明银屑病皮损的菌株和亚菌株水平分析。
Tett等人先前完成的一项研究招募了银屑病患者，这些患者的皮肤微生物组样本取自耳朵和肘部的银屑病斑块以及未受银屑病影响的皮肤部位。他们进行了全基因组鸟枪法测序，并将其数据集公开。我们使用CosmosID-HUB微生物组分析流程分析了该数据集，以评估菌株和亚菌株分类学分析以及功能基因谱。
使用CosmosID分析流程进行分析时，与未受影响的区域相比，受影响区域的耳朵和肘部部位的α多样性均降低。受影响部位的葡萄球菌属和棒状杆菌属的相对丰度增加。我们鉴定出了酵母马拉色菌的区分物种和菌株，包括在健康肘部样本中显著富集的。银屑病皮肤中不存在维生素B12产生基因，而在健康样本中存在，这支持了银屑病斑块中相对维生素B12缺乏的观点。噬菌体分析显示，未受影响的肘部样本中与葡萄球菌相关的噬菌体多样性更高。
本研究中鉴定出的微生物菌株及其功能作用的更多样性可能有助于为银屑病量身定制治疗方案。
