Doulidis P G, Reisner R, Auer A, Dimmel Katharina, Lammer Thomas, Künzel F
Clinical Unit of Internal Medicine Small Animals, Department for Companion Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
Department of Pathobiology, Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria.
Front Vet Sci. 2024 Sep 5;11:1461136. doi: 10.3389/fvets.2024.1461136. eCollection 2024.
Bocaviruses are single-stranded DNA viruses from the family, which have been minimally discussed in veterinary literature and are considered potentially pathogenic. Due to the recurring illness among young dogs in a closed cohort of military dogs in Austria, we assessed the prevalence, possible disease manifestation and outcome of CBoV-2 infection in this cohort.
This led to a comprehensive study that not only analyzed past cases but also performed prospective screening PCR tests to identify CBoV-2 positive dogs within this specific dog population. Pharyngeal and rectal swabs were taken. In addition, a control group ( = 20) of clinically healthy client-owned dogs was sampled. A total of 190 samples were taken and tested for the presence of CBoV-2 specific nucleic acid using screening PCR. In addition to the primers used for routine diagnostics, two other primer pairs were used to verify questionable results. The retrospective part of the study includes a total of 13 military dogs that had previously shown suspected clinical signs.
At the time of the first examination within the prospective part of the study, CBoV-2 was detected in 31% (12/39) of the dogs. During the second examination, 2% (1/47) tested positive, while all PCR testing in the control group ( = 20) was negative in all cases. The retrospective evaluation of the 13 cases revealed a total of six animals tested positive for CBoV-2 via screening PCR. All puppies suffered from skin lesions (papules, vesicles, or pustules). Other clinical signs included diarrhea (83%), vomiting (77%), respiratory (15%), and neurological (8%) signs.
According to the study there are certain indications that CBoV-2 shares similarities with CPV-2 infection but also exhibits critical differences, making their differentiation essential for patient management, outcomes, and prevention strategies.
博卡病毒是细小病毒科的单链DNA病毒，在兽医文献中讨论较少，被认为具有潜在致病性。由于奥地利一个封闭的军犬群体中的幼犬反复患病，我们评估了该群体中犬博卡病毒2型（CBoV-2）感染的患病率、可能的疾病表现及结果。
这促成了一项全面的研究，该研究不仅分析既往病例，还进行前瞻性筛查PCR检测，以在这个特定犬群中识别CBoV-2阳性犬。采集咽拭子和直肠拭子。此外，对20只临床健康的客户拥有的犬组成的对照组进行采样。共采集190份样本，使用筛查PCR检测CBoV-2特异性核酸的存在。除了用于常规诊断的引物外，还使用另外两对引物来验证可疑结果。该研究的回顾性部分包括总共13只先前表现出疑似临床症状的军犬。
在该研究前瞻性部分的首次检查时，31%（12/39）的犬检测出CBoV-2。在第二次检查时，2%（1/47）检测呈阳性，而对照组（n = 20）的所有PCR检测在所有情况下均为阴性。对这13例病例的回顾性评估显示，共有6只动物通过筛查PCR检测出CBoV-2呈阳性。所有幼犬都有皮肤病变（丘疹、水疱或脓疱）。其他临床症状包括腹泻（83%）、呕吐（77%）、呼吸道症状（15%）和神经症状（8%）。
根据该研究，有某些迹象表明CBoV-2与犬细小病毒2型（CPV-2）感染有相似之处，但也存在关键差异，这使得它们的区分对于患者管理、结果和预防策略至关重要。
