Kakumu S, Yoshioka K, Tsubouchi A
Gastroenterol Jpn. 1985 Jun;20(3):216-21. doi: 10.1007/BF02774707.
A double-antibody radioimmunoassay was developed to detect antibody to human liver-specific membrane lipoprotein (anti-LSP antibody) in patients' serum. Anti-human LSP monoclonal antibody was labeled with 125I and anti-anti-LSP antiserum raised in rabbits was used as the first antibody in the assay. Anti-LSP antibody level was quantitatively measured and the assay was shown to be specific. Anti-LSP antibody was found in 5/8 patients with type B acute viral hepatitis (AVH), 3/7 patients with type A AVH, 1/6 patients with non-A, non-B AVH, 10/17 patients with chronic active hepatitis (CAH), 6/16 patients with chronic persistent hepatitis, 13/16 patients with active cirrhosis of the liver and 7/19 patients with primary nonhepatic autoimmune diseases such as glomerulonephritis, systemic lupus erythematosus and rheumatoid arthritis. The mean levels of anti-LSP were increased in patients with cirrhosis of the liver (p less than 0.01), CAH (p less than 0.05) and AVH (p less than 0.05) when compared with that of normal individuals. However, the frequencies of anti-LSP did not depend on HBsAg status. The data showed that anti-LSP antibody can be detected without the use of LSP preparation although it is also found in patients with primary nonhepatic autoimmune diseases.
已开发出一种双抗体放射免疫测定法，用于检测患者血清中针对人肝特异性膜脂蛋白的抗体（抗LSP抗体）。用人抗LSP单克隆抗体用125I标记，并使用在兔中产生的抗抗LSP抗血清作为该测定法中的第一抗体。定量测量抗LSP抗体水平，结果表明该测定法具有特异性。在8例B型急性病毒性肝炎（AVH）患者中有5例、7例A型AVH患者中有3例、6例非甲非乙型AVH患者中有1例、17例慢性活动性肝炎（CAH）患者中有10例、16例慢性持续性肝炎患者中有6例、16例活动性肝硬化患者中有13例以及19例原发性非肝脏自身免疫性疾病（如肾小球肾炎、系统性红斑狼疮和类风湿性关节炎）患者中有7例检测到抗LSP抗体。与正常个体相比，肝硬化患者（p<0.01）、CAH患者（p<0.05）和AVH患者（p<0.05）的抗LSP平均水平升高。然而，抗LSP的频率并不取决于HBsAg状态。数据表明，尽管在原发性非肝脏自身免疫性疾病患者中也发现了抗LSP抗体，但无需使用LSP制剂即可检测到抗LSP抗体。
