College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135628. doi: 10.1016/j.ijbiomac.2024.135628. Epub 2024 Sep 18.
In this study, to investigate the potential for delivering bioactive substances, various cereal proteoglycan nanoparticles were prepared using anti-solvent precipitation for the delivery of oyster protein hydrolysate (OPH). The hydrogen bonding and hydrophobic interaction were the driving forces for OPH embedding. OPH-Loaded Zein/NaCas/Gum Arabic composite nanoparticles could self-assemble into spherical (191 nm) particles with strong stability, uniform distribution (about PDI = 0.2), and high negative charge (about -28 mV). The secondary structure of OPH-Loaded zein was converted from α-helix to random coil with heat treatment. The off-flavor, including (E, E)-2,4-heptadienal, octadecane, and 1-octanol in OPH-Loaded Zein nanoparticles significantly decreased. Furthermore, these nanoparticles containing OPH had excellent oxidation resistance, in vitro digestibility, and non-toxicity in Caco-2 and HT-29 cells. Overall, this work provides an efficient strategy for OPH delivery, suggesting that OPH could be ingested as a nutraceutical in powder form while also promoting the development of high-flavor-quality oyster products.
在这项研究中，为了研究递送生物活性物质的潜力，使用抗溶剂沉淀法制备了各种谷物蛋白聚糖纳米粒子，用于递送牡蛎蛋白水解物（OPH）。氢键和疏水相互作用是 OPH 嵌入的驱动力。牡蛎蛋白水解物负载的玉米醇溶蛋白/NaCas/阿拉伯胶复合纳米粒子可以自组装成具有强稳定性、均匀分布（约 PDI = 0.2）和高负电荷（约-28 mV）的球形（191 nm）颗粒。热处理后，OPH 负载玉米醇溶蛋白的二级结构从α-螺旋转变为无规卷曲。OPH 负载玉米醇溶蛋白纳米粒子中的不良风味物质，包括（E，E）-2,4-庚二烯醛、十八烷和 1-辛醇，明显减少。此外，这些含有 OPH 的纳米粒子具有优异的抗氧化性、体外消化性和在 Caco-2 和 HT-29 细胞中的非毒性。总的来说，这项工作为 OPH 的递送提供了一种有效的策略，表明 OPH 可以以粉末形式作为营养保健品摄入，同时也促进了高风味品质牡蛎产品的开发。
