Guizhou Academy of Forestry, Guiyang, Guizhou, 550005, China.
Institute for Forest Resources and Environment of Guizhou, Guizhou University, Guiyang, 550025, China.
BMC Genomics. 2024 Sep 20;25(1):887. doi: 10.1186/s12864-024-10795-0.
Camellia oleifera is an important woody oil tree in China, in which the flowers and fruits appear during the same period. The endogenous hormone changes and transcription expression levels in different parts of the flower tissue (sepals, petals, stamens, and pistils), flower buds, leaves, and seeds of Changlin 23 high-yield (H), Changlin low-yield (L), and control (CK) C. oleifera groups were studied. The abscisic acid (ABA) content in the petals and stamens in the L group was significantly higher than that in the H and CK groups, which may be related to flower and fruit drops. The high N6-isopentenyladenine (iP) and indole acetic acid (IAA) contents in the flower buds may be associated with a high yield. Comparative transcriptome analysis showed that the protein phosphatase 2C (PP2C), jasmonate-zim-domain protein (JAZ), and WRKY-related differentially expressed genes (DEGs) may play an important role in determining leaf color. Gene set enrichment analysis (GSEA) comparison showed that jasmonic acid (JA) and cytokinin play an important role in determining the pistil of the H group. In this study, endogenous hormone and transcriptome analyses were carried out to identify the factors influencing the large yield difference in C. oleifera in the same year, which provides a theoretical basis for C. oleifera in the future.
油茶是中国重要的木本油料树种，其花和果实同期出现。本研究以高产的长林 23 号（H）、低产的长林 23 号（L）和对照（CK）油茶组为材料，分析了花组织（花萼、花瓣、雄蕊和雌蕊）、花蕾、叶片和种子中内源激素变化和转录表达水平。L 组花瓣和雄蕊中的脱落酸（ABA）含量明显高于 H 组和 CK 组，这可能与花果脱落有关。高的 N6-异戊烯腺嘌呤（iP）和吲哚乙酸（IAA）含量可能与高产量有关。比较转录组分析表明，蛋白磷酸酶 2C（PP2C）、茉莉酸-zim 结构域蛋白（JAZ）和 WRKY 相关差异表达基因（DEGs）可能在决定叶片颜色中起重要作用。基因集富集分析（GSEA）比较表明，茉莉酸（JA）和细胞分裂素在决定 H 组雌蕊中起重要作用。本研究通过内源激素和转录组分析，鉴定了油茶当年产量差异大的影响因素，为今后油茶的研究提供了理论依据。
