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序列型 35 的基因组流行病学揭示了种内和种间克隆传播。

Genomic epidemiology of sequence type 35 reveals intraspecies and interspecies clonal transmission.

机构信息

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.

School of Laboratory Medicine, Hangzhou Medical College, Zhejiang, People's Republic of China.

出版信息

Emerg Microbes Infect. 2024 Dec;13(1):2408322. doi: 10.1080/22221751.2024.2408322. Epub 2024 Sep 30.

DOI:10.1080/22221751.2024.2408322
PMID:39305009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11443556/
Abstract

sequence type (ST) 35 has been found in humans and animals worldwide. However, its genomic epidemiology and clonal transmission have not been explored in detail. In this study, 176 ST35 isolates from six countries were sequenced. Genomic diversity, clonal transmission and epidemiological data were analyzed. Sporulation and virulence capacities were measured. Four ribotypes (RT) were identified including RT046 (97.2%), RT656 (1.1%), RT427 (0.6%), and RT AI-78 (1.1%). Phylogenetic analysis of 176 ST35 genomes, along with 50 publicly available genomes, revealed two distinctive lineages without time-, region-, or source-dependent distribution. However, the distribution of antimicrobial resistance genes differed significantly between the two lineages. Nosocomial and communal transmission occurred in humans with the isolates differed by ≤ two core-genome single-nucleotide polymorphism (cgSNPs) and clonal circulation was found in pigs with the isolates differed by ≤ four cgSNPs. Notably, interspecies clonal transmission was identified among three patients with community acquired infection and pigs with epidemiological links, differed by ≤ nine cgSNPs. Toxin B (TcdB) concentrations were significantly higher in human isolates compared to pig isolates, and ST35 isolates exhibited stronger sporulation capacities than other STs. Our study provided new genomic insights and epidemiological evidence of ST35 intraspecies and interspecies clonal transmission, which can also be facilitated by its strong sporulation capacity.

摘要

序列类型 35（ST35）已在全球人类和动物中发现。然而，其基因组流行病学和克隆传播尚未得到详细探讨。在本研究中，对来自六个国家的 176 株 ST35 分离株进行了测序。分析了基因组多样性、克隆传播和流行病学数据。测量了孢子形成和毒力能力。鉴定了四个核糖型（RT），包括 RT046（97.2%）、RT656（1.1%）、RT427（0.6%）和 RT AI-78（1.1%）。对 176 株 ST35 基因组以及 50 株公开可用基因组进行的系统发育分析表明，存在两个没有时间、区域或来源依赖性分布的独特谱系。然而，两种谱系之间的抗生素耐药基因分布有显著差异。在人类中发生了医院和社区传播，分离株之间相差不超过两个核心基因组单核苷酸多态性（cgSNP）；在猪中发现了克隆循环，分离株之间相差不超过四个 cgSNP。值得注意的是，在具有流行病学联系的三名社区获得性感染患者和猪之间，发现了种间克隆传播，分离株之间相差不超过九个 cgSNP。人类分离株的毒素 B（TcdB）浓度明显高于猪分离株，ST35 分离株的孢子形成能力强于其他 ST。我们的研究提供了 ST35 种内和种间克隆传播的新基因组见解和流行病学证据，其强大的孢子形成能力也可促进其传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/f6f1a74fc828/TEMI_A_2408322_F0004_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/1820c690197b/TEMI_A_2408322_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/d77aaa6fdb97/TEMI_A_2408322_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/879c86b0b730/TEMI_A_2408322_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/f6f1a74fc828/TEMI_A_2408322_F0004_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/1820c690197b/TEMI_A_2408322_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/d77aaa6fdb97/TEMI_A_2408322_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/879c86b0b730/TEMI_A_2408322_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/206c/11443556/f6f1a74fc828/TEMI_A_2408322_F0004_OB.jpg

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