CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, UC-Biotech, BiocantPark, Núcleo 04, Lote 8, 3060-197 Cantanhede, Portugal; CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, UC-Biotech, BiocantPark, Núcleo 04, Lote 8, 3060-197 Cantanhede, Portugal.
Marine Microbiology Group, Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), Esporles, Spain.
Syst Appl Microbiol. 2024 Nov;47(6):126553. doi: 10.1016/j.syapm.2024.126553. Epub 2024 Sep 17.
One hundred and sixty-three extreme halophiles were recovered from a single sample collected from an inland solar saltern in Rio Maior. Based on random amplified polymorphic DNA (RAPD) profiles and partial 16S rRNA gene sequencing 125 isolates were identified as members of the Archaea domain within the genus Halorubrum. Two strains, RMP-11 and RMP-47, showed 99.1 % sequence similarity with the species Halorubrum californiense based on phylogenetic analysis of the 16S rRNA gene sequence. However, phylogenetic analysis based on five housekeeping genes, atpB, EF-2, glnA, ppsA and rpoB', showed Halorubrum coriense as the closest related species with 96.7 % similarity. The average nucleotide identity (ANI) of strains RMP-11, RMP-47 and species Hrr. coriense were within the range of 90.0-90.5 %, supporting that strains RMP-11 and RMP-47 represent a novel species of the genus Halorubrum. These strains formed red-pigmented colonies that were able to grow in a temperature range of 25-50 °C. Polyhydroxyalkanoate (PHA) granules were detected in both strains. The polar lipid profile was identical to the neutrophilic species of the genus Halorubrum. The Rio Maior sample from which both strains were isolated was metagenome sequenced. We identified five metagenome-assembled genomes representing novel Halorubrum species but distinct from the species represented by strains RMP-11 and RMP-47. Based on phylogenetic, phylogenomic, comparative genomics, physiological and chemotaxonomic parameters, we describe a new species of the genus Halorubrum represented by strains RMP-11 (=CECT 30760 = DSM 115521) and RMP-47 (=CECT 30761 = DSM 115541) for which we propose the name Halorubrum miltondacostae sp. nov.
从里奥马约尔的一个内陆太阳能盐场采集的单一样本中回收了 163 种极端嗜盐菌。基于随机扩增多态性 DNA (RAPD) 图谱和部分 16S rRNA 基因测序，125 个分离株被鉴定为古菌域盐杆菌属的成员。根据 16S rRNA 基因序列的系统发育分析，两个菌株 RMP-11 和 RMP-47 与加利福尼亚盐杆菌种的序列相似度为 99.1%。然而，基于 5 个看家基因(atpB、EF-2、glnA、ppsA 和 rpoB')的系统发育分析表明，盐红杆菌种是与 RMP-11 和 RMP-47 最接近的相关种，相似度为 96.7%。菌株 RMP-11、RMP-47 和 Hrr. coriense 的平均核苷酸同一性 (ANI) 均在 90.0-90.5%的范围内，支持菌株 RMP-11 和 RMP-47 代表盐杆菌属的一个新种。这些菌株形成红色色素的菌落，能够在 25-50°C 的温度范围内生长。在这两个菌株中都检测到聚羟基烷酸 (PHA) 颗粒。极性脂谱与盐杆菌属的嗜中性种相同。从分离这两个菌株的里奥马约尔样本中进行了宏基因组测序。我们鉴定了五个宏基因组组装基因组，代表了新的盐杆菌属物种，但与菌株 RMP-11 和 RMP-47 所代表的物种不同。基于系统发育、系统基因组学、比较基因组学、生理和化学分类学参数，我们描述了一种新的盐杆菌属物种，代表菌株 RMP-11(=CECT 30760=DSM 115521)和 RMP-47(=CECT 30761=DSM 115541)，我们提议将其命名为盐杆菌 miltondacostae 新种。
