Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Life Science, Linyi University, Linyi 276005, China.
Xuzhou Institute of Agricultural Sciences, Xuzhou 221131, China.
Food Chem. 2025 Jan 15;463(Pt 1):141142. doi: 10.1016/j.foodchem.2024.141142. Epub 2024 Sep 5.
Contamination with pesticides has inflicted substantial harm on human health; therefore, developing rapid, ultra-sensitive, and non-labelling simultaneous detection methods for multiple pesticides is necessary. In this study, we demonstrated that α-hemolysin (α-HL) nanopore sensor can detect and discriminate organophosphorus pesticides of phoxim and omethoate in a single nanopore without requiring labels of the probes or purification of the pesticides in real samples. Aptamers specifically recognise and bind pesticides to obtain pesticide-aptamer complexes that produce characteristic current signals while passing through the nanopore. Phoxim and omethoate were accurately distinguished by a portable instrument within minutes, and their detection sensitivity was up to the femtomole level, whether detected alone or simultaneously. The detection limits of phoxim and omethoate were 8.13 × 10 M and 4.16 × 10 M. The recoveries of phoxim and omethoate from pear, tomato, and cucumber samples were 82.0-107.0 % and 81.9-118.3 % respectively, with coefficient of variable below 8.0 %.
农药污染对人类健康造成了严重危害；因此，开发快速、超灵敏、无标记的多种农药同时检测方法是必要的。在本研究中，我们证明了α-溶血素（α-HL）纳米孔传感器可以在单个纳米孔中检测和区分膦和氧乐果等有机磷农药，而无需探针标记或实际样品中农药的纯化。适体特异性识别和结合农药，以获得农药-适体复合物，当它们通过纳米孔时产生特征电流信号。在几分钟内，便携式仪器就能准确区分膦和氧乐果，其检测灵敏度可达飞摩尔水平，无论是单独检测还是同时检测。膦和氧乐果的检测限分别为 8.13×10^-10 M 和 4.16×10^-10 M。从梨、番茄和黄瓜样品中检测到的膦和氧乐果的回收率分别为 82.0-107.0%和 81.9-118.3%，变异系数低于 8.0%。
