Zhu Xuchun, Zhang Xiaoyue, Wang Ziyuan, Ren Feiyue, Zhu Xuyou, Chen Bingyu, Liu Hongzhi, Wuyun Tana
School of Food and Health, Beijing Technology and Business University, Beijing 100080, China.; Chinese Academy of Forestry Research Institute of Non-Timber Forestry, Zhengzhou 450000, China.
School of Food and Health, Beijing Technology and Business University, Beijing 100080, China.
Food Chem. 2025 Jan 15;463(Pt 2):141336. doi: 10.1016/j.foodchem.2024.141336. Epub 2024 Sep 17.
In today's social environment, the objective reality of people's increasing life pressure, environmental deterioration, and enhanced ultraviolet rays caused by the destruction of the ozone layer has led to the aggravation of people's oxidative stress. Therefore, exogenous antioxidant peptides have become a hot topic in research. In the context of insufficient protein supply and resource recycling, almond meal was used as raw material in this study. As a by-product of oil processing, it has a protein content of 68 % and antioxidant-related amino acids accounted for 84.62 %, which can be used as a high-quality natural source of antioxidant peptides. Taking antioxidant activity as the only indicator, papain was screened as a hydrolase, and 7 antioxidant peptides such as YLSF, LPSYVN and SPHWNVN were separated and purified. The affinity energy of docking with Keap1-Nrf2-ARE protein molecules was -7.5--8.9 kal/mol, and hydrophobic stacking, hydrogen bonding and intermolecular forces were maintained. Seven antioxidant peptides were synthesized in solid phase, and the IC values of in vitro ABTS scavenging rates were 3.59 μg/mL-6.73 μg/mL, and the antioxidant capacity was stronger than that of glutathione and ascorbic acid. In the in vitro cellular ROS scavenging capacity, all seven peptides had the effect of scavenging intracellular ROS, among which YLSF and ESWNPRDPQF had stronger scavenging capacity than glutathione. Finally, the mouse skin staining method determined that apricot antioxidant peptides had a significant inhibitory effect on UV damage to mouse skin, and targeted proteomics was used to clarify that apricot antioxidant peptides inhibited UV damage by mainly affecting three pathways, including the base excision repair pathway. This study not only improved the economic value of processing by-products, but also obtained 7 highly active almond antioxidant peptides, tapping the potential ability of apricot antioxidant peptides to be incorporated into functional food or cosmetic formulations.
在当今社会环境下，人们生活压力增大、环境恶化以及臭氧层破坏导致紫外线增强的客观现实，使得人们的氧化应激加剧。因此，外源性抗氧化肽成为研究热点。在蛋白质供应不足和资源循环利用的背景下，本研究以杏仁粕为原料。作为油脂加工的副产品，其蛋白质含量为68%，抗氧化相关氨基酸占84.62%，可作为优质的天然抗氧化肽来源。以抗氧化活性为唯一指标，筛选木瓜蛋白酶作为水解酶，分离纯化出YLSF、LPSYVN和SPHWNVN等7种抗氧化肽。与Keap1-Nrf2-ARE蛋白分子对接的亲和能为-7.5--8.9千卡/摩尔，并维持疏水堆积、氢键和分子间作用力。固相合成7种抗氧化肽，体外ABTS清除率的IC值为3.59微克/毫升-6.73微克/毫升，抗氧化能力强于谷胱甘肽和抗坏血酸。在体外细胞ROS清除能力方面，7种肽均有清除细胞内ROS的作用，其中YLSF和ESWNPRDPQF的清除能力强于谷胱甘肽。最后，通过小鼠皮肤染色法确定杏仁抗氧化肽对小鼠皮肤紫外线损伤有显著抑制作用，并采用靶向蛋白质组学阐明杏仁抗氧化肽主要通过影响碱基切除修复途径等三条途径抑制紫外线损伤。本研究不仅提高了加工副产品的经济价值，还获得了7种高活性杏仁抗氧化肽，挖掘了杏仁抗氧化肽应用于功能性食品或化妆品配方的潜在能力。
