Fan Hekai, Li Ruoxuan, Zhang Yanbing, Xu Xiaoyun, Pan Siyi, Liu Fengxia
College of Food Science and Technology, Huazhong agricultural university, Wuhan, Hubei, PR China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan, Hubei, PR China.
College of Food Science and Technology, Huazhong agricultural university, Wuhan, Hubei, PR China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan, Hubei, PR China.
Int J Biol Macromol. 2024 Sep 20;280(Pt 3):135843. doi: 10.1016/j.ijbiomac.2024.135843.
In this work, three degraded polysaccharides (DMPP-40, DMPP-60, DMPP-80) were successfully obtained by HO/ascorbic acid degradation and gradient ethanol precipitation from Satsuma mandarin peel pectin (MPP), and their physicochemical properties, antioxidant and prebiotic activities were investigated. The molecular weight of MPP, DMPP-40, DMPP-60, DMPP-80 were determined to be 336.83 ± 10.57, 18.93 ± 0.54, 26.07 ± 0.83 and 8.71 ± 0.27 kDa, respectively. The ethanol concentration significantly affected the physicochemical properties of DMPPs. DMPP-60 showed the highest yield (69.07 %) and uronic acid content (64.85 %), DMPP-80 showed the lowest molecular weight (8.71 kDa), and the composition and proportion of monosaccharides of DMPPs were significantly different. Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (H NMR) confirmed that DMPPs exhibited similar functional groups, while X-ray diffraction (XRD) indicated that DMPP-40 possessed some crystallographic sequences. Scanning electron microscopy (SEM) images directly verified the fragmented structure and reduced surface area of DMPPs. Besides, the HO/ascorbic acid treatment could obviously reduce the apparent viscosity and thermal stability of MPP. Meanwhile, the results of bioactivity assay showed that DMPPs possessed better antioxidant activity and probiotics pro-proliferative effects compared with MPP. DMPP-80 could significantly inhibit lipopolysaccharides (LPS)-stimulated production of inflammatory factors (including nitric oxide (NO), interleukin (IL)-6, tumor necrosis factor (TNF)-α and interleukin (IL)-1β) in RAW264.7 cells. Results suggest that the HO/ascorbic acid combined with gradient ethanol precipitation has potential applications in degradation and separation of MPP to improve its biological activities.
在本研究中，通过HO/抗坏血酸降解和梯度乙醇沉淀法，成功从温州蜜柑皮果胶（MPP）中获得了三种降解多糖（DMPP-40、DMPP-60、DMPP-80），并对其理化性质、抗氧化和益生元活性进行了研究。测定MPP、DMPP-40、DMPP-60、DMPP-80的分子量分别为336.83±10.57、18.93±0.54、26.07±0.83和8.71±0.27 kDa。乙醇浓度显著影响DMPPs的理化性质。DMPP-60的产率最高（69.07%），糖醛酸含量最高（64.85%），DMPP-80的分子量最低（8.71 kDa），且DMPPs的单糖组成和比例存在显著差异。傅里叶变换红外光谱（FT-IR）和核磁共振光谱（H NMR）证实DMPPs具有相似的官能团，而X射线衍射（XRD）表明DMPP-40具有一些晶体学序列。扫描电子显微镜（SEM）图像直接证实了DMPPs的碎片化结构和表面积减小。此外，HO/抗坏血酸处理可明显降低MPP的表观粘度和热稳定性。同时，生物活性测定结果表明，与MPP相比，DMPPs具有更好的抗氧化活性和益生菌增殖促进作用。DMPP-80可显著抑制RAW264.7细胞中脂多糖（LPS）刺激的炎症因子（包括一氧化氮（NO）、白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β）的产生。结果表明，HO/抗坏血酸结合梯度乙醇沉淀法在MPP的降解和分离以提高其生物活性方面具有潜在应用价值。
