Wang Xueyan, Ding Yi, Zhang Xueping, Feng Yichao, Li Chenglin, Ge Yichen, Yang Yaosen, Su Jianqing, Chu Xiuling
College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China.
College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China.
Poult Sci. 2025 Apr;104(4):104933. doi: 10.1016/j.psj.2025.104933. Epub 2025 Feb 21.
The aim of this study was to evaluate the effect of degraded polysaccharide of Acanthopanax senticosus (ASPS-1) on the immunological effects and appropriate dosage of broiler chicks with a view to developing a new feed additive. For the experimental design, 180 broiler chicks were randomly divided into six groups, ASPS-1 low, medium and high dose groups, undegraded Acanthopanax senticosus polysaccharide (ASPS) low and medium dose groups and blank control group. The drug was administered for 21 consecutive days, and the growth and data of immune organ index and immune factors were recorded on the 7th, 14th and 21st d. Finally, the effect of ASPS-1 on the intestinal flora of broiler chicks was investigated by high-throughput sequencing of the 16S rRNA gene and the correlation between the main flora and intestinal indexes was analyzed, and the function of microbial community was predicted by using PICRUSt2. The results showed that the addition of high dose of ASPS-1 could promote the body weight growth of broiler chicks, had no significant effect on immune organs, significantly promoted the increase of intestinal villi and crypt ratio, and effectively regulated the levels of serum antioxidant factors and immune indexes. Analysis of the intestinal flora showed that ASPS-1H promoted the proliferation of Lactobacillus, Faecalibacterium, Negativibacillus, and Eubacterium and inhibited the colonization of Desulfovibrio and Turicibacter, and that proliferation of Faecalibacterium, Negativibacillus and Eubacterium was associated with the development of intestinal villi. Predictive analysis of PICRUSt2 function indicates that proliferation of Lactobacillus, Faecalibacterium, Negativibacillus and Eubacterium functions through amino acid metabolism, global and overview maps, replication and repair pathways function. In summary, the addition of high doses of ASPS-1 can improve the immunity of broilers and has the potential to be used as a feed additive.
本研究旨在评估刺五加降解多糖（ASPS-1）对肉鸡免疫效果及适宜剂量的影响，以期开发一种新型饲料添加剂。在实验设计中，180只肉鸡随机分为6组，即ASPS-1低、中、高剂量组，未降解刺五加多糖（ASPS）低、中剂量组和空白对照组。连续给药21天，分别在第7、14和21天记录生长情况、免疫器官指数和免疫因子数据。最后，通过16S rRNA基因高通量测序研究ASPS-1对肉鸡肠道菌群的影响，分析主要菌群与肠道指标之间的相关性，并使用PICRUSt2预测微生物群落功能。结果表明，高剂量ASPS-1添加可促进肉鸡体重增长，对免疫器官无显著影响，显著促进肠道绒毛和隐窝比值增加，并有效调节血清抗氧化因子和免疫指标水平。肠道菌群分析显示，ASPS-1H促进了乳酸杆菌、粪杆菌、阴性杆菌和真杆菌的增殖，抑制了脱硫弧菌和Turicibacter的定植，且粪杆菌、阴性杆菌和真杆菌的增殖与肠道绒毛发育相关。PICRUSt2功能预测分析表明，乳酸杆菌、粪杆菌、阴性杆菌和真杆菌的增殖通过氨基酸代谢、全局和概览图谱、复制和修复途径发挥作用。综上所述，高剂量ASPS-1添加可提高肉鸡免疫力，具有作为饲料添加剂的潜力。
