State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China.
The Second People's Hospital of Anhui Province, Anhui, Hefei, China; Affiliated Hospital of Anhui Medical College, Anhui, Hefei, China.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135861. doi: 10.1016/j.ijbiomac.2024.135861. Epub 2024 Sep 20.
Inulin has been reported to alleviate colitis. In this study, colitis patients' feces were used to simulate fermentation to demonstrate changes in the microbiota profile in the presence of inulin. We found inulin can reshape the gut microbiota profile of colitis patients, especially by altering the abundance of Faecalibacterium and Blautia. Interestingly, the subsequent co-culture with inulin demonstrated that inulin promoted the growth of these two strains of bacteria. The dextran sodium sulfate (DSS)-induced mouse model was used to examine the effect of inulin and its combination with two probiotics on colitis. Results showed that all three treatments can alleviate the clinical symptoms, including weight-losing, colon-shortening, and the Disease Activity Index (DAI) score. Further investigations showed that the administrations regulate colitis mice's pro- and anti-inflammatory cytokines, such as TNF-α, IL-1β, IL-6, IL-10, and IL-17. Also, they alter the relative abundance of Faecalibacterium and Blautia, change the short-chain fatty acids (SCFAs) profile in the cecum and colon, and improve the intestinal barrier; specifically, the intervention increased the expressions of Claudin, Occludin, Zonula Occludens (ZO)-1, and Mucin (MUC)-2 in colonic tissues, thus restoring the colonic tissue structure and morphology of colitis mice. Collectively, our results confirm that inulin can alter the colitis patients' characteristic microbial community, and they can ameliorate experimental colitis by inhibiting the TRL4/MyD88/NF-κB signaling pathway-improving the inflammatory response and enhancing the intestinal barrier. In conclusion, we propose that inulin may hold promise as a functional food therapeutic approach for the treatment of colitis.
菊粉已被报道可缓解结肠炎。在这项研究中，我们使用结肠炎患者的粪便进行发酵模拟，以展示菊粉存在时微生物群特征的变化。我们发现菊粉可以重塑结肠炎患者的肠道微生物群特征，特别是通过改变粪杆菌和布劳特氏菌的丰度。有趣的是，随后与菊粉的共培养表明菊粉促进了这两种细菌的生长。我们使用葡聚糖硫酸钠（DSS）诱导的小鼠模型来研究菊粉及其与两种益生菌组合对结肠炎的影响。结果表明，三种处理方法都可以缓解临床症状，包括体重减轻、结肠缩短和疾病活动指数（DAI）评分。进一步的研究表明，这些处理方法调节了结肠炎小鼠的促炎和抗炎细胞因子，如 TNF-α、IL-1β、IL-6、IL-10 和 IL-17。此外，它们还改变了粪杆菌和布劳特氏菌的相对丰度，改变了盲肠和结肠中的短链脂肪酸（SCFA）谱，并改善了肠道屏障；具体来说，干预增加了结肠组织中 Claudin、Occludin、Zonula Occludens（ZO）-1 和 Mucin（MUC）-2 的表达，从而恢复了结肠炎小鼠的结肠组织结构和形态。总之，我们的结果证实菊粉可以改变结肠炎患者的特征性微生物群落，并通过抑制 TLR4/MyD88/NF-κB 信号通路改善炎症反应和增强肠道屏障来缓解实验性结肠炎。总之，我们提出菊粉可能作为一种功能性食品治疗方法，用于治疗结肠炎。
