Osman Nadia, Adawi Diya, Molin Göran, Ahrne Siv, Berggren Anna, Jeppsson Bengt
Dept of Surgery University Hospital Malmö, Lund University, 205 02 Malmö, Sweden.
BMC Gastroenterol. 2006 Oct 28;6:31. doi: 10.1186/1471-230X-6-31.
Pathogenesis of inflammatory bowel disease is thought to be through different factors and there is a relationship between the gut flora and the risk of its development. Probiotics can manipulate the microflora in chronic inflammation and may be effective in treating inflammation. Bifidobacterium are saccharolytic and their growth in the gut can be promoted by non-absorbable carbohydrates and its increase in the colon appears to be of benefit.
Oligofructose and inulin (OFI) alone and the two B. infantis DSM 15158 and DSM 15159 with and without OFI, were fed to Sprague-Dawley rats for 7 days prior to colitis induction and administrations continued for another 7 days with the DSS. Colitis severity assessed using a Disease Activity Index. Samples were collected 7 days after colitis induction, for intestinal bacterial flora, bacterial translocation, short chain fatty acids (SCFAs), myeloperoxidase (MPO), cytokines (IL-1beta, TNF-alpha, IL-10 and TGF-beta) and malondialdehyde (MDA).
OFI alone or the B. infantis strains with and without OFI improved significantly the DAI and decreased colonic MPO activity. Colonic tissue IL-1beta decreased significantly in all treated groups except B. infantis DSM 15158. MDA decreased significantly in B. infantis DSM 15159 with and without OFI compared to colitis control. Succinic acid increased significantly in OFI group with and without DSM 15159 compared to all groups. Sum values of propionic, succinic acid and butyric acid increased significantly in all groups compare to the colitis control. Bacterial translocation to mesenteric lymph nodes decreased significantly in all groups compared to colitis control. Translocation to the liver decreased significantly in all groups compare to the colitis control and OFI + B. infantis DSM 15158 groups.
Administrations of OFI and Bifidobacterium improve DSS-induced acute colitis and have an anti-inflammatory effect. Major differences in effect were observed between the two B. infantis strains as indicated in MDA and succinic acid concentration as well as bacterial translocation rate in synbiotic combinations.
炎症性肠病的发病机制被认为是由多种不同因素导致的，并且肠道菌群与其发病风险之间存在关联。益生菌可调控慢性炎症中的微生物群落，可能对治疗炎症有效。双歧杆菌具有分解糖类的能力，其在肠道内的生长可被不可吸收的碳水化合物所促进，且其在结肠内数量的增加似乎有益。
在诱导结肠炎前7天，将低聚果糖和菊粉（OFI）单独以及与两株婴儿双歧杆菌DSM 15158和DSM 15159（有无OFI）分别喂给Sprague-Dawley大鼠，并用葡聚糖硫酸钠（DSS）继续给药7天。使用疾病活动指数评估结肠炎严重程度。在诱导结肠炎7天后采集样本，检测肠道细菌菌群、细菌易位、短链脂肪酸（SCFAs）、髓过氧化物酶（MPO）、细胞因子（IL-1β、TNF-α、IL-10和TGF-β）以及丙二醛（MDA）。
单独使用OFI或使用有无OFI的婴儿双歧杆菌菌株均显著改善了疾病活动指数，并降低了结肠MPO活性。除婴儿双歧杆菌DSM 15158外，所有治疗组的结肠组织IL-1β均显著降低。与结肠炎对照组相比，有无OFI的婴儿双歧杆菌DSM 15159组的MDA均显著降低。与所有组相比，有无DSM 15159的OFI组中琥珀酸均显著增加。与结肠炎对照组相比，所有组中丙酸、琥珀酸和丁酸的总和值均显著增加。与结肠炎对照组相比，所有组中细菌向肠系膜淋巴结的易位均显著减少。与结肠炎对照组以及OFI + 婴儿双歧杆菌DSM 15158组相比，所有组中细菌向肝脏的易位均显著减少。
给予OFI和双歧杆菌可改善DSS诱导的急性结肠炎并具有抗炎作用。如在合生元组合中的MDA和琥珀酸浓度以及细菌易位率所示，两株婴儿双歧杆菌菌株在效果上存在主要差异。
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