Mojica Jacqueline, Arévalo Valentina, Juarez Jose G, Galarza Ximena, Gonzalez Karla, Carrazco Andrés, Suazo Harold, Harris Eva, Coloma Josefina, Ponce Patricio, Balmaseda Angel, Cevallos Varsovia
Department of Entomology, Sustainable Sciences Institute, Managua, Nicaragua.
Centro de Investigación en Enfermedades Infecciosas y Vectoriales, Instituto Nacional de Investigación en Salud Pública, Quito, Ecuador.
J Med Entomol. 2025 Jan 13;62(1):220-224. doi: 10.1093/jme/tjae121.
Aedes mosquitoes, as vectors of medically important arthropod-borne viruses (arboviruses), constitute a major public health threat that requires entomological and epidemiological surveillance to guide vector control programs to prevent and reduce disease transmission. In this study, we present the collaborative effort of 1 year of Aedes ageypti (Linnaeus, 1762) mosquito-based arbovirus surveillance in 2 geographically distinct regions of Latin America (Nicaragua and Ecuador). Adult female mosquitoes were collected using backpack aspirators in over 2,800 randomly selected households (Nicaragua, Ecuador) and 100 key sites (Nicaragua) from 8 distinct communities (Nicaragua: 2, Ecuador: 6). A total of 1,358 mosquito female pools were processed for RNA extraction and viral RNA detection using real-time reverse transcription-polymerase chain reaction. Ten positive dengue virus (DENV) pools were detected (3 in Nicaragua and 7 in Ecuador), all of which were found during the rainy season and matched the serotypes found in humans (Nicaragua: DENV-1 and DENV-4; Ecuador: DENV-2). Infection rates ranged from 1.13 to 23.13, with the Nicaraguan communities having the lowest infection rates. Our results demonstrate the feasibility of detecting DENV-infected Aedes mosquitoes in low-resource settings and underscore the need for targeted mosquito arbovirus sampling and testing, providing valuable insights for future surveillance programs in the Latin American region.
伊蚊作为重要医学节肢动物传播病毒（虫媒病毒）的传播媒介，构成了重大的公共卫生威胁，需要进行昆虫学和流行病学监测，以指导病媒控制项目，预防和减少疾病传播。在本研究中，我们展示了在拉丁美洲两个地理上不同的地区（尼加拉瓜和厄瓜多尔）开展的为期1年的基于埃及伊蚊（林奈，1762年）的虫媒病毒监测的合作成果。使用背包式吸虫器在8个不同社区（尼加拉瓜2个，厄瓜多尔6个）中随机选择的2800多个家庭（尼加拉瓜、厄瓜多尔）和100个重点地点（尼加拉瓜）收集成年雌性蚊子。总共对1358个雌蚊混合样本进行了处理，用于RNA提取和使用实时逆转录-聚合酶链反应进行病毒RNA检测。检测到10个登革病毒（DENV）阳性混合样本（尼加拉瓜3个，厄瓜多尔7个），所有这些样本均在雨季发现，并且与在人类中发现的血清型相匹配（尼加拉瓜：DENV-1和DENV-4；厄瓜多尔：DENV-2）。感染率在1.13至23.13之间，尼加拉瓜社区的感染率最低。我们的结果证明了在资源匮乏地区检测感染DENV的伊蚊的可行性，并强调了针对性的蚊虫虫媒病毒采样和检测的必要性，为拉丁美洲地区未来的监测项目提供了有价值的见解。
