• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

厄瓜多尔埃斯梅拉达斯农村地区登革热病毒2型基因型III传播的昆虫病毒学监测及基因组洞察

Entomo-Virological Surveillance and Genomic Insights into DENV-2 Genotype III Circulation in Rural Esmeraldas, Ecuador.

作者信息

Carrazco-Montalvo Andrés, Gutiérrez-Pallo Diana, Arévalo Valentina, Ponce Patricio, Rodríguez-Polit Cristina, Echeverría-Garcés Gabriela, Coloma Josefina, Nipaz Victoria, Cevallos Varsovia

机构信息

Centro de Referencia Nacional de Genómica, Secuenciación y Bioinformática (GENSBIO), Instituto Nacional de Investigación en Salud Pública (INSPI), Quito 170403, Ecuador.

Centro de Investigación en Enfermedades Infecciosas y Vectoriales (CIREV), Instituto Nacional de Investigación en Salud Pública (INSPI), Quito 170403, Ecuador.

出版信息

Pathogens. 2025 May 28;14(6):541. doi: 10.3390/pathogens14060541.

DOI:10.3390/pathogens14060541
PMID:40559549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12195760/
Abstract

Ecuador, a tropical country with frequent dengue outbreaks, including a surge from 16,017 cases in 2022 to 61,329 in 2024, was the focus of this study. The study was conducted in Borbon, a semi-urban rural town in the Esmeraldas province. Genomic analysis, alongside entomo-virological surveillance, provides valuable insights into DENV-2 genotypes. Five pools of female mosquitoes from Borbon tested positive for DENV serotype 2 through RT-qPCR. One positive pool (CT = 16.13) was sequenced using Illumina MiSeq, and genotyping was conducted via the Dengue Typing Tool and Maximum Likelihood phylogenetic tree. The genotype assigned was III Southern Asian-American. Comparison with other genomes revealed genetic similarity to a human dengue genome sequenced in 2021, also from Esmeraldas, clustering with genomes reported across the Americas, particularly from Colombia and Venezuela. This study enhances our understanding of dengue virus epidemiology in rural areas, emphasizing the critical role of clinical case surveillance and vector monitoring in guiding evidence-based interventions.

摘要

厄瓜多尔是一个热带国家，登革热疫情频发，病例数从2022年的16017例激增至2024年的61329例，该国是本研究的重点。研究在埃斯梅拉达斯省的一个半城郊镇博尔邦进行。基因组分析与昆虫病毒学监测一起，为登革病毒2型基因型提供了有价值的见解。通过逆转录定量聚合酶链反应（RT-qPCR）检测，来自博尔邦的五组雌性蚊子登革病毒血清型2呈阳性。使用Illumina MiSeq对一个阳性样本池（CT = 16.13）进行测序，并通过登革热分型工具和最大似然系统发育树进行基因分型。确定的基因型为III南亚-美洲型。与其他基因组的比较显示，其与2021年在埃斯梅拉达斯测序的一个人类登革热基因组具有遗传相似性，与美洲各地报告的基因组聚类，特别是来自哥伦比亚和委内瑞拉的基因组。本研究增进了我们对农村地区登革热病毒流行病学的理解，强调了临床病例监测和病媒监测在指导循证干预措施方面的关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/9ad5ad61b875/pathogens-14-00541-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/a2d1027c8238/pathogens-14-00541-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/470d644bc071/pathogens-14-00541-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/9ad5ad61b875/pathogens-14-00541-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/a2d1027c8238/pathogens-14-00541-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/470d644bc071/pathogens-14-00541-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea1d/12195760/9ad5ad61b875/pathogens-14-00541-g003.jpg

相似文献

1
Entomo-Virological Surveillance and Genomic Insights into DENV-2 Genotype III Circulation in Rural Esmeraldas, Ecuador.厄瓜多尔埃斯梅拉达斯农村地区登革热病毒2型基因型III传播的昆虫病毒学监测及基因组洞察
Pathogens. 2025 May 28;14(6):541. doi: 10.3390/pathogens14060541.
2
Whole Genome Sequencing of DENV-2 isolated from mosquitoes in Esmeraldas, Ecuador. Genomic epidemiology of genotype III Southern Asian-American in the country.从厄瓜多尔埃斯梅拉达斯的蚊子中分离出的登革热病毒2型全基因组测序。该国南亚-美洲基因型III的基因组流行病学。
bioRxiv. 2024 Feb 8:2024.02.06.579255. doi: 10.1101/2024.02.06.579255.
3
DENV-2 Circulation and Host Preference Among Highly Anthropophilic, Outdoor-Biting in Dar es Salaam, Tanzania.坦桑尼亚达累斯萨拉姆嗜人血、户外叮咬的蚊子中登革热病毒2型的传播及宿主偏好性
Viruses. 2025 Jun 5;17(6):818. doi: 10.3390/v17060818.
4
Sticky traps for Aedes aegypti surveillance and targeted vector control in Sincelejo, Colombia.用于哥伦比亚辛塞莱霍埃及伊蚊监测和目标性病媒控制的粘性诱捕器。
Biomedica. 2025 Mar 28;45(1):118-132. doi: 10.7705/biomedica.7290.
5
Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines.菲律宾塔拉克市采集的与疑似人类病例同时发生的埃及伊蚊个体中登革热病毒的监测。
Parasit Vectors. 2020 Nov 25;13(1):594. doi: 10.1186/s13071-020-04470-y.
6
Natural vertical transmission of dengue virus in Aedes aegypti and Aedes albopictus: a systematic review.自然垂直传播登革病毒在埃及伊蚊和白纹伊蚊：系统评价。
Parasit Vectors. 2018 Feb 1;11(1):77. doi: 10.1186/s13071-018-2643-9.
7
Virological Surveillance of Aedes aegypti Vectors Identifies All Four Dengue Serotypes in a Hyperendemic Region.登革热病毒学监测表明在高度流行地区伊蚊传播了全部四种血清型。
Ecohealth. 2022 Mar;19(1):75-84. doi: 10.1007/s10393-022-01583-x. Epub 2022 Mar 22.
8
Dengue in the Middle East and North Africa: A Systematic Review.中东和北非地区的登革热：一项系统评价。
PLoS Negl Trop Dis. 2016 Dec 7;10(12):e0005194. doi: 10.1371/journal.pntd.0005194. eCollection 2016 Dec.
9
Assessment of a multiplex arbovirus PCR Detection Test in an area endemic for Chikungunya, Zika, and Dengue viruses: An evaluation of kit performance characteristics in line with Clinical Laboratory Improvement Amendments (CLIA) Standards.在基孔肯雅病毒、寨卡病毒和登革热病毒流行地区对多重虫媒病毒PCR检测试验的评估：按照临床实验室改进修正案（CLIA）标准对试剂盒性能特征的评估。
PLoS One. 2025 Jun 24;20(6):e0309626. doi: 10.1371/journal.pone.0309626. eCollection 2025.
10
Global Epidemiology of Dengue Outbreaks in 1990-2015: A Systematic Review and Meta-Analysis.1990 - 2015年登革热疫情的全球流行病学：系统评价与荟萃分析
Front Cell Infect Microbiol. 2017 Jul 12;7:317. doi: 10.3389/fcimb.2017.00317. eCollection 2017.

本文引用的文献

1
Characterizing dengue transmission in rural areas: A systematic review.描述农村地区登革热传播情况：系统综述。
PLoS Negl Trop Dis. 2023 Jun 8;17(6):e0011333. doi: 10.1371/journal.pntd.0011333. eCollection 2023 Jun.
2
Genetic Analysis of Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh, in 2018-2022.2018-2022 年孟加拉国达卡重症和非重症登革热病例的病毒基因分析。
Viruses. 2023 May 10;15(5):1144. doi: 10.3390/v15051144.
3
Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil.
系统进化重建揭示了新型登革热病毒 1V 分支和登革热病毒 2 基因型 III 在巴西东北部的循环和持续存在。
Viruses. 2023 Apr 28;15(5):1073. doi: 10.3390/v15051073.
4
Phylogenetic Analysis of Transmission Dynamics of Dengue in Large and Small Population Centers, Northern Ecuador.厄瓜多尔北部大、小城市中心登革热传播动力学的系统进化分析。
Emerg Infect Dis. 2023 May;29(5):888-897. doi: 10.3201/eid2905.221226.
5
Genomic Sequencing of Dengue Virus Strains Associated with Papua New Guinean Outbreaks in 2016 Reveals Endemic Circulation of DENV-1 and DENV-2.2016 年巴布亚新几内亚疫情相关登革热病毒株的基因组测序揭示了 DENV-1 和 DENV-2 的本地流行。
Am J Trop Med Hyg. 2022 Jul 5;107(6):1234-1238. doi: 10.4269/ajtmh.21-1292. Print 2022 Dec 14.
6
Global Evolutionary History and Dynamics of Dengue Viruses Inferred from Whole Genome Sequences.从全基因组序列推断登革病毒的全球进化历史和动态。
Viruses. 2022 Mar 28;14(4):703. doi: 10.3390/v14040703.
7
ViralFlow: A Versatile Automated Workflow for SARS-CoV-2 Genome Assembly, Lineage Assignment, Mutations and Intrahost Variant Detection.ViralFlow：一种用于 SARS-CoV-2 基因组组装、谱系分配、突变和宿主内变异检测的通用自动化工作流程。
Viruses. 2022 Jan 23;14(2):217. doi: 10.3390/v14020217.
8
A dengue outbreak in a rural community in Northern Coastal Ecuador: An analysis using unmanned aerial vehicle mapping.厄瓜多尔北部沿海农村社区的登革热疫情：利用无人机测绘进行的分析。
PLoS Negl Trop Dis. 2021 Sep 27;15(9):e0009679. doi: 10.1371/journal.pntd.0009679. eCollection 2021 Sep.
9
Augur: a bioinformatics toolkit for phylogenetic analyses of human pathogens.奥古：用于人类病原体系统发育分析的生物信息学工具包。
J Open Source Softw. 2021;6(57). doi: 10.21105/joss.02906. Epub 2021 Jan 7.
10
Dengue Vaccines: The Promise and Pitfalls of Antibody-Mediated Protection.登革热疫苗：抗体介导保护的前景与挑战。
Cell Host Microbe. 2021 Jan 13;29(1):13-22. doi: 10.1016/j.chom.2020.12.011.