Detection of from rodents residing in the densely populated residential regions along the coastal areas of Banyuwangi Sub District, Indonesia.

BACKGROUND

Extensive attention has been devoted to studies of in rodents ever since it became recognised as a zoonotic pathogen known as atypical human trypanosomiasis. Regrettably, although infections of small mammals remain significant public health concerns for humans, there is a lack of comprehensive study in Indonesia.

AIM

The aim of the study was to detect from rodents residing in the densely populated residential regions along the coastal areas of Banyuwangi Sub District.

METHODS

A total of 169 rodents were captured across three villages of Kampung Mandar, Lateng and Kepatihan, using rat single live traps. After being euthanized and identified, the blood samples were collected from each rodent via cardiac puncture. Subsequently, the samples were subjected to native (direct blood microscopic examination), microscopic blood smear examination, and molecular analyses utilizing TRYP1S-TRYP1R (623 bp) and LEW1S-LEW1R (220 bp).

RESULTS

The results demonstrated that two species of rodents were successfully captured: (65.68%) and (34.32%). Based on the native and microscopic blood smear examinations, the prevalence of across three villages was 23.08% and 24.26% for molecular analysis employing both primers, respectively. The highest prevalence was found in Kampung Mandar Village (31.18%), followed by Kepatihan (16.67%) and Lateng Villages (15.71%).

CONCLUSION

Statistical analysis revealed that was more prevalent in compared to . In terms of sex, no statistically significant distinction was observed between female and male infected rodents of either species ( > 0.05), indicating both species can serve as a source of for humans in the surveyed villages.