哈萨克斯坦犬群中流感病毒的循环（2023-2024 年）。

Circulation of influenza viruses in the dog population in Kazakhstan (2023-2024).

机构信息

The Research and Production Center for Microbiology and Virology, Almaty, Republic of Kazakhstan.

Kazakh National Agrarian University, Almaty, Republic of Kazakhstan.

出版信息

Open Vet J. 2024 Aug;14(8):1896-1904. doi: 10.5455/OVJ.2024.v14.i8.17. Epub 2024 Aug 31.

DOI:10.5455/OVJ.2024.v14.i8.17

PMID:39308731

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11415905/

Abstract

BACKGROUND

Dogs in close contact with humans can serve as a source of potentially dangerous reassortant influenza viruses (IVs) with zoonotic potential. The dog's body can serve as a vessel for the emergence of new IVs. These new viruses can become a source of infection for other animals and humans. The potential for zoonotic transmission of IVs from dogs to humans poses a public health risk.

AIM

Study of the circulation of IVs in the dog population in Almaty, Kazakhstan.

METHODS

Biosamples (oropharyngeal swabs and blood serum) from dogs were collected from veterinary clinics in Almaty in 2023-2024. Samples were screened using RT-PCR, HI assay, and ELISA.

RESULTS

RT-PCR analysis of 355 nasopharyngeal swabs showed the presence of influenza A virus (IAV) in 32 samples (9.01% of the total number of samples analyzed). When subtyping IAV H1N1 RNA was detected in 19 swabs (5.35%). IAV subtype could not be determined in 13 PCR-positive samples (3.66%). The genetic material of IAV H3N2, H5, H7, and H9, as well as coronavirus, bocavirus, and adenovirus has not been identified. In a serological analysis of 180 blood sera using ELISA, antibodies to IAV were detected in 5.56% ( = 10). The results of the HI assay showed the presence of antihemagglutinins to A/H1N1pdm in 6.11% (11 samples), to A/H3N2 in 9.44% (17 samples), and no antibodies to IAV H5, H7, and type B were detected.

CONCLUSION

There is no information about human infection with any canine influenza virus. However, many cases of infection in dogs with human IAVs H1N1, H1N1pdm09, and H3N2 have been described. When dogs are co-infected with different IAVs, new recombinant IAVs may emerge that can infect humans and other animals. Therefore, ongoing global surveillance of animal populations is necessary to monitor the evolution and circulation of viruses dangerous to public health. This is also important for timely preparation for the emergence of a new zoonotic influenza virus that has pandemic potential for humans.

摘要

背景

与人类密切接触的狗可以成为具有人畜共患潜力的潜在危险重组流感病毒 (IVs) 的来源。狗的身体可以成为新 IVs 出现的容器。这些新病毒可能成为其他动物和人类感染的来源。IVs 从狗传播给人类的人畜共患传播的可能性对公共卫生构成威胁。

目的

研究哈萨克斯坦阿拉木图的狗群中 IVs 的循环情况。

方法

2023-2024 年，从阿拉木图的兽医诊所采集狗的生物样本（咽拭子和血清）。使用 RT-PCR、HI 测定和 ELISA 对样本进行筛查。

结果

对 355 份鼻咽拭子的 RT-PCR 分析显示，在 32 份样本（分析的总样本数的 9.01%）中存在甲型流感病毒 (IAV)。在 19 份拭子中检测到 IAV H1N1 RNA（5.35%）。在 13 份 PCR 阳性样本中无法确定 IAV 亚型（3.66%）。未鉴定出 IAV H3N2、H5、H7 和 H9 的遗传物质，以及冠状病毒、 bocavirus 和腺病毒。使用 ELISA 对 180 份血血清进行血清学分析，发现 5.56%（=10）存在针对 IAV 的抗体。HI 测定结果显示，在 6.11%（11 份样本）中存在针对 A/H1N1pdm 的抗血凝素，在 9.44%（17 份样本）中存在针对 A/H3N2 的抗血凝素，未检测到针对 IAV H5、H7 和 B 型的抗体。

结论

没有关于人类感染任何犬流感病毒的信息。然而，已经描述了许多狗感染人类 IAV H1N1、H1N1pdm09 和 H3N2 的病例。当狗同时感染不同的 IAV 时，可能会出现新的重组 IAV，从而感染人类和其他动物。因此，有必要对动物种群进行持续的全球监测，以监测对公共卫生构成威胁的病毒的演变和传播。这对于及时准备具有人类大流行潜力的新人畜共患流感病毒也很重要。