Mendoza Hector, Lamb Emma A, Thomas Joshua, Tavares Derica Goncalves, Schroeder Luke A, Müller Christian, Agrawal Nisha, Schirawski Jan, Perlin Michael H
Department of Biology, Program on Disease Evolution, University of Louisville, Louisville, KY, United States.
Matthias Schleiden Institute - Genetics, Friedrich-Schiller University Jena, Jena, Germany.
Front Physiol. 2024 Sep 6;15:1264359. doi: 10.3389/fphys.2024.1264359. eCollection 2024.
Modern understanding of the concept of genetic diversity must include the study of both nuclear and organellar DNA, which differ greatly in terms of their structure, organization, gene content and distribution. This study comprises an analysis of the genetic diversity of the smut fungus f. sp. from a mitochondrial perspective.
Whole-genome sequencing data was generated from biological samples of collected from different geographical regions. Multiple sequence alignment and gene synteny analysis were performed to further characterize genetic diversity in the context of mitogenomic polymorphisms.
Mitochondria of strains collected in China contained unique sequences. The largest unique sequence stretch encompassed a portion of , a mitochondrial gene encoding one of the subunits that make up complex IV of the mitochondrial electron transport chain. This unique sequence had high percent identity to the mitogenome of the related species and .
The results of this study hint at potential horizontal gene transfer or mitochondrial genome recombination events during the evolutionary history of basidiomycetes. Additionally, the distinct polymorphic region detected in the Chinese mitogenome provides the ideal foundation to develop a diagnostic method to discern between mitotypes and enhance knowledge on the genetic diversity of this organism.
现代对遗传多样性概念的理解必须包括对核DNA和细胞器DNA的研究，它们在结构、组织、基因含量和分布方面有很大差异。本研究从线粒体角度分析了黑粉菌f. sp.的遗传多样性。
从不同地理区域收集的生物样本中生成全基因组测序数据。进行了多序列比对和基因共线性分析，以在有丝分裂基因组多态性的背景下进一步表征遗传多样性。
在中国收集的菌株线粒体含有独特序列。最大的独特序列片段包含一部分，一个线粒体基因编码构成线粒体电子传递链复合物IV的亚基之一。这个独特序列与相关物种和的有丝分裂基因组有很高的百分比同一性。
本研究结果暗示在担子菌进化历史中可能存在水平基因转移或线粒体基因组重组事件。此外，在中国有丝分裂基因组中检测到的独特多态性区域为开发一种区分有丝分裂类型的诊断方法和增强对该生物体遗传多样性的认识提供了理想基础。
