Huo Xiang, Tan Zhongming, Qian Huimin, Qin Yuanfang, Dong Chen, Li Chuchu, Kong Xiaoxiao, Hong Jie
National Health Commission (NHC) Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, People's Republic of China.
Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, People's Republic of China.
Infect Drug Resist. 2024 Sep 16;17:4001-4009. doi: 10.2147/IDR.S454042. eCollection 2024.
is a significant etiological agent of infection and commonly inhabits the human nasopharynx, alongside other potentially pathogenic bacteria. In this study, strains were obtained from a community population and subjected to investigation of their phenotypes, genotypes, and vaccine coverage.
was isolated from nasopharyngeal swab samples of a healthy population in the Guangfu Community. Capsular serotypes and genotypes were identified using Quellung reaction and multilocus sequence typing (MLST), respectively. The antimicrobial susceptibility was tested using minimum inhibitory concentrations.
In total, 500 unvaccinated people were sampled. Ninety-four strains were identified. Common serotypes were 19F, 6A, and 9V. The strain coverages of PCV13 and PPV23 were 61.7% and 58.5%, respectively. About 27.6% isolates were non-susceptible to penicillin, and over 80% were resistant to erythromycin and doxycycline. Among 27 novel sequence types (STs) identified in all strains, the most common STs were ST236 (6/94, 6.4%) and ST12669 (6/94, 6.4%). Nearly half of the strains were grouped into four clone complexes (CC12665, CC271, CC6011, and CC180), of which CC271 showed the highest resistance to PEN.
In our study, various drug-resistant clone complexes of were found in the healthy population, the elderly, and children. Consequently, pneumococcal vaccines should be included in the national immunization schedule to prevent disease spread.
是一种重要的感染病原体，通常与其他潜在病原菌一起栖息于人类鼻咽部。在本研究中，从社区人群中获取菌株，并对其表型、基因型和疫苗覆盖率进行调查。
从广福社区健康人群的鼻咽拭子样本中分离出。分别使用荚膜肿胀反应和多位点序列分型（MLST）鉴定荚膜血清型和基因型。使用最低抑菌浓度测试抗菌药敏性。
总共对500名未接种疫苗的人进行了采样。鉴定出94株菌株。常见血清型为19F、6A和9V。PCV13和PPV23的菌株覆盖率分别为61.7%和58.5%。约27.6%的分离株对青霉素不敏感，超过80%对红霉素和强力霉素耐药。在所有菌株中鉴定出的27种新序列类型（STs）中，最常见的STs是ST236（6/94，6.4%）和ST12669（6/94，6.4%）。近一半的菌株被归入四个克隆复合体（CC12665、CC271、CC6011和CC180），其中CC271对青霉素的耐药性最高。
在我们的研究中，在健康人群、老年人和儿童中发现了各种耐药克隆复合体。因此，肺炎球菌疫苗应纳入国家免疫规划以预防疾病传播。
