Jung Seunghun, Kim Sangil, Shin Seunggwan
School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Research Institute of Basic Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Zookeys. 2024 Sep 12;1212:1-15. doi: 10.3897/zookeys.1212.117845. eCollection 2024.
Over the past decade, the prevalence of mass outbreaks involving non-native insects has sparked concerns about their potential negative impact on human inhabited areas and local environments. Duda, 1933 (Diptera, Bibionidae) was recently recognized as an invasive pest in South Korea, causing public nuisance through mass outbreaks in the Seoul Metropolitan Area during early summer. In this study, we present the first complete mitochondrial genome of , generated from the PacBio HiFi long-read sequencing data. Notably, the length of the circular genome is found to be larger than any annotated reference sequences of mitochondrial genomes for the infraorder Bibionomorpha, which is attributable to an unusually long A+T rich control region. We conducted a phylogenetic analysis of Bibionomorpha, focusing specifically on the family Bibionidae, using nearly all available mitochondrial genome data to elucidate relationships among genera within Bibionidae. Our phylogeny of Bibionomorpha recovered a strong monophyly of the family Bibionidae and its three subfamilies: Bibioninae ( + ), Hesperininae ( + ), and Pleciinae (), corroborating the recently proposed taxonomic classification system of Bibionidae. Furthermore, we discuss evolutionary trends within Bibionidae based on our well-supported higher relationships of the superfamily Bibionoidea.
在过去十年中，涉及非本地昆虫的大规模爆发事件日益普遍，引发了人们对其可能对人类居住区域和当地环境造成负面影响的担忧。1933年发现的Duda（双翅目，毛蚊科）最近被确认为韩国的一种入侵害虫，在初夏期间于首尔大都市区大量爆发，给公众带来困扰。在本研究中，我们展示了首个基于PacBio HiFi长读长测序数据生成的完整线粒体基因组。值得注意的是，发现该环状基因组的长度大于毛蚊亚目线粒体基因组的任何已注释参考序列，这归因于一个异常长的富含A+T的控制区域。我们对毛蚊亚目进行了系统发育分析，特别关注毛蚊科，使用了几乎所有可用的线粒体基因组数据来阐明毛蚊科各属之间的关系。我们构建的毛蚊亚目系统发育树恢复了毛蚊科及其三个亚科的强烈单系性：毛蚊亚科（+）、黄昏毛蚊亚科（+）和大角毛蚊亚科（），证实了最近提出的毛蚊科分类系统。此外，我们基于双翅总科得到充分支持的高级关系，讨论了毛蚊科内部的进化趋势。
