Jia Xiaoli, Li Mingzhe, Zhang Qi, Jia Miao, Hong Lei, Zhang Shuqi, Wang Yuhua, Luo Yangxin, Wang Tingting, Ye Jianghua, Wang Haibin
College of Tea and Food, Wuyi University, Wuyishan, China.
College of Life Science, Longyan University, Longyan, China.
Front Plant Sci. 2024 Sep 6;15:1444436. doi: 10.3389/fpls.2024.1444436. eCollection 2024.
Dahongpao mother tree ( (L.) O. Ktze) is a representative of Wuyi rock tea. Whether there is a difference in rhizosphere soil microbial diversity and function between asexually propagated cuttings of Dahongpao (PD) and the parent Dahongpao mother tree (MD) has not been reported. In this study, high throughput sequencing technology was used to analyze rhizosphere soil microbial diversity, functions and their relationship with soil available nutrients and enzyme activities in MD and PD. The results showed that available nitrogen, phosphorus and potassium contents and urease, protease, acid phosphatase and sucrase activities of rhizosphere soils in MD were significantly higher than those in PD. Both bacterial and fungal diversity were higher in rhizosphere soils in MD than in PD, and secondly, the bacterial community structure was less stable while the fungal community structure was more stable in PD compared to MD. There were significant differences between MD and PD tea tree rhizosphere soils in 6 genera of characteristic bacteria and 4 genera of characteristic fungi. The results of function and interaction effect analysis showed that the rhizosphere soil available nutrient content and enzyme activities in MD were significantly higher than those in PD, and their contributions mainly originated from and of characteristic bacteria and of characteristic fungi. Secondly, MD maybe had a stronger ability to inhibit soil pathogens than PD, with the main contribution coming from and of characteristic fungi. Overall, compared with PD, soil texture in MD was relatively better, and its soil nutrient cycling-related enzyme activities were stronger, which was more favorable to soil nutrient cycling and increased the available nutrient content of the soil, which in turn promoted the growth of tea trees. This study provides an important reference for the planting and management of tea tree cuttings and microbial regulation of tea tree growth.
大红袍母树（[茶树学名]）是武夷岩茶的代表。大红袍无性繁殖扦插苗（PD）与母树大红袍（MD）根际土壤微生物多样性和功能是否存在差异尚未见报道。本研究采用高通量测序技术分析MD和PD根际土壤微生物多样性、功能及其与土壤有效养分和酶活性的关系。结果表明，MD根际土壤有效氮、磷、钾含量及脲酶、蛋白酶、酸性磷酸酶和蔗糖酶活性均显著高于PD。MD根际土壤细菌和真菌多样性均高于PD，其次，与MD相比，PD中细菌群落结构稳定性较差，而真菌群落结构稳定性较好。MD和PD茶树根际土壤在6属特征细菌和4属特征真菌上存在显著差异。功能及相互作用效应分析结果表明，MD根际土壤有效养分含量和酶活性显著高于PD，其贡献主要源于特征细菌的[具体细菌种类或类别1]和[具体细菌种类或类别2]以及特征真菌的[具体真菌种类或类别]。其次，MD对土壤病原菌的抑制能力可能比PD更强，主要贡献来自特征真菌的[具体真菌种类或类别1]和[具体真菌种类或类别2]。总体而言，与PD相比，MD的土壤质地相对较好，其与土壤养分循环相关的酶活性较强，更有利于土壤养分循环并增加土壤有效养分含量，进而促进茶树生长。本研究为茶树扦插种植与管理及茶树生长的微生物调控提供了重要参考。
