Ye Jianghua, Wang Yuhua, Lin Shaoxiong, Wang Yuchao, Chen Pengyuan, Hong Lei, Jia Xiaoli, Kang Jiaqian, Wu Zeyan, Wang Haibin
College of Tea and Food, Wuyi University, Wuyishan, China.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Plant Sci. 2023 Feb 24;14:1137465. doi: 10.3389/fpls.2023.1137465. eCollection 2023.
Acidification can seriously affect the growth of tea trees and the yield and quality of tea leaves. In this study, we analyzed the effects of acidification on the physicochemical properties, microorganisms and metabolites of tea rhizosphere soils with different pH values, and the results showed that with the increase of soil pH, the organic matter content, cation exchange capacity, microbial biomass carbon, microbial biomass nitrogen, microbial respiration intensity, bacterial number and actinomyces number in tea rhizosphere soil all showed an increasing trend, while the fungi number decreased. The results of soil metabolite analysis showed that 2376, 2377 and 2359 metabolites were detected in tea rhizosphere soil with pH values of 3.29, 4.74 and 5.32, respectively, and the number of similar compounds reached 2331, accounting for more than 98%. The results of soil metabolite content analysis showed that with the increase of soil pH, the total contents of metabolite of tea rhizosphere soil increased significantly. The results of correlation analysis between physicochemical indexes of soil and microorganisms and soil metabolites showed that physicochemical indexes of soil and microorganisms were significantly correlated with 221 soil metabolites, among which 55 were significantly positively correlated and 166 were significantly negatively correlated. Based on correlation interaction network analysis, 59 characteristic compounds were obtained and divided into 22 categories, among which 7 categories compounds showed a significant increasing trend with the increase of soil pH, while the other 15 categories compounds showed the opposite trend. Based on the functional analysis of characteristic metabolites, this study found that with the increase of soil pH in tea rhizosphere, the diversity and number of soil microorganisms increased, and the cyclic ability of C and N of tea rhizosphere soil was enhanced, which in turn might lead to the enhancement of resistance of tea tree and promote the growth of tea tree.
酸化会严重影响茶树生长以及茶叶的产量和品质。在本研究中，我们分析了酸化对不同pH值茶树根际土壤的理化性质、微生物和代谢产物的影响，结果表明，随着土壤pH值升高，茶树根际土壤中的有机质含量、阳离子交换量、微生物生物量碳、微生物生物量氮、微生物呼吸强度、细菌数量和放线菌数量均呈增加趋势，而真菌数量减少。土壤代谢产物分析结果显示，pH值为3.29、4.74和5.32的茶树根际土壤中分别检测到2376、2377和2359种代谢产物，相似化合物数量达2331种，占比超过98%。土壤代谢产物含量分析结果表明，随着土壤pH值升高，茶树根际土壤代谢产物总含量显著增加。土壤理化指标与微生物及土壤代谢产物的相关性分析结果显示，土壤理化指标和微生物与221种土壤代谢产物显著相关，其中55种呈显著正相关，166种呈显著负相关。基于相关性相互作用网络分析，获得59种特征化合物并分为22类，其中7类化合物随着土壤pH值升高呈显著增加趋势，而其他15类化合物呈相反趋势。基于特征代谢产物的功能分析，本研究发现随着茶树根际土壤pH值升高，土壤微生物多样性和数量增加，茶树根际土壤碳、氮循环能力增强，进而可能导致茶树抗性增强并促进茶树生长。
