• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蛋白质组学和生物信息学研究表明，FGF8及相关枢纽基因的过表达与卵巢癌进展和预后不良相关。

Proteomics and Bioinformatics Investigations Link Overexpression of FGF8 and Associated Hub Genes to the Progression of Ovarian Cancer and Poor Prognosis.

作者信息

Kumar Vikrant, Tomar Anil Kumar, Thapliyal Ayushi, Yadav Savita

机构信息

Department of Biophysics All India Institute of Medical Sciences, New Delhi 11029, India.

出版信息

Biochem Res Int. 2024 Sep 13;2024:4288753. doi: 10.1155/2024/4288753. eCollection 2024.

DOI:10.1155/2024/4288753
PMID:39309198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11415250/
Abstract

Ovarian cancer's asymptomatic nature, high recurrence rate, and resistance to platinum-based chemotherapy highlight the need to find and characterize new diagnostic and therapeutic targets. While prior studies have linked aberrant expression of fibroblast growth factor 8 (FGF8) to various cancer types, its precise role has remained elusive. Recently, we observed that FGF8 silencing reduces the cancer-promoting properties of ovarian cancer cells, and thus, this study aimed to understand how FGF8 regulates the development of ovarian cancer. LC-MS/MS-based quantitative proteomics analysis identified 418 DEPs, and most of them were downregulated in FGF8-silenced ovarian cancer cells. Many of these DEPs are associated with cancer progression and unfavorable prognosis. To decipher the biological significance of DEPs, bioinformatics analyses encompassing gene ontology, pathway analysis, protein-protein interaction networks, and expression analysis of hub genes were carried out. Hub genes identified in the FGF8 protein network were upregulated in ovarian cancer compared to controls and were linked to poor prognosis. Subsequently, the expression of hub genes was correlated with patient survival and regulation of the tumor microenvironment. Conclusively, FGF8 and associated hub genes help in the progression of ovarian cancer, and their overexpression may lead to higher immune infiltration, poor prognosis, and poor survival.

摘要

卵巢癌的无症状特性、高复发率以及对铂类化疗的耐药性凸显了寻找和鉴定新的诊断及治疗靶点的必要性。虽然先前的研究已将成纤维细胞生长因子8（FGF8）的异常表达与多种癌症类型联系起来，但其确切作用仍不清楚。最近，我们观察到FGF8沉默会降低卵巢癌细胞的促癌特性，因此，本研究旨在了解FGF8如何调节卵巢癌的发展。基于液相色谱-串联质谱的定量蛋白质组学分析鉴定出418个差异表达蛋白（DEP），其中大多数在FGF8沉默的卵巢癌细胞中表达下调。这些DEP中的许多与癌症进展和不良预后相关。为了解析DEP的生物学意义，我们进行了包括基因本体论、通路分析、蛋白质-蛋白质相互作用网络以及枢纽基因表达分析在内的生物信息学分析。与对照组相比，在FGF8蛋白网络中鉴定出的枢纽基因在卵巢癌中上调，并且与不良预后相关。随后，枢纽基因的表达与患者生存率以及肿瘤微环境的调节相关。总之，FGF8及相关枢纽基因促进卵巢癌进展，它们的过表达可能导致更高的免疫浸润、不良预后和较差的生存率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/87690a37efba/BRI2024-4288753.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/a1b04534a1d5/BRI2024-4288753.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/b9bd4aefb932/BRI2024-4288753.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/c6e1fe7d07da/BRI2024-4288753.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/3b83e048f530/BRI2024-4288753.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/a54e40ff4ae1/BRI2024-4288753.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/1a42b71e783a/BRI2024-4288753.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/f7847cc417cb/BRI2024-4288753.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/ec738826af5f/BRI2024-4288753.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/87690a37efba/BRI2024-4288753.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/a1b04534a1d5/BRI2024-4288753.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/b9bd4aefb932/BRI2024-4288753.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/c6e1fe7d07da/BRI2024-4288753.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/3b83e048f530/BRI2024-4288753.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/a54e40ff4ae1/BRI2024-4288753.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/1a42b71e783a/BRI2024-4288753.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/f7847cc417cb/BRI2024-4288753.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/ec738826af5f/BRI2024-4288753.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbf/11415250/87690a37efba/BRI2024-4288753.009.jpg

相似文献

1
Proteomics and Bioinformatics Investigations Link Overexpression of FGF8 and Associated Hub Genes to the Progression of Ovarian Cancer and Poor Prognosis.蛋白质组学和生物信息学研究表明，FGF8及相关枢纽基因的过表达与卵巢癌进展和预后不良相关。
Biochem Res Int. 2024 Sep 13;2024:4288753. doi: 10.1155/2024/4288753. eCollection 2024.
2
Unveiling the Significance of FGF8 Overexpression in Orchestrating the Progression of Ovarian Cancer.揭示 FGF8 过表达在调控卵巢癌进展中的意义。
Int J Mol Sci. 2023 Sep 18;24(18):14239. doi: 10.3390/ijms241814239.
3
Identification of prognosis-related hub genes of ovarian cancer through bioinformatics analyses and experimental verification.通过生物信息学分析和实验验证鉴定卵巢癌预后相关的枢纽基因。
Medicine (Baltimore). 2022 Sep 9;101(36):e30374. doi: 10.1097/MD.0000000000030374.
4
Integrated bioinformatics analysis for the screening of hub genes and therapeutic drugs in ovarian cancer.卵巢癌中枢纽基因和治疗药物的筛选的综合生物信息学分析。
J Ovarian Res. 2020 Jan 27;13(1):10. doi: 10.1186/s13048-020-0613-2.
5
Identification of the Hub Genes Associated with the Prognosis of Ovarian Cancer Patients via Integrated Bioinformatics Analysis and Experimental Validation.通过综合生物信息学分析和实验验证鉴定与卵巢癌患者预后相关的枢纽基因
Cancer Manag Res. 2021 Jan 26;13:707-721. doi: 10.2147/CMAR.S282529. eCollection 2021.
6
Platinum-resistance in epithelial ovarian cancer: an interplay of epithelial-mesenchymal transition interlinked with reprogrammed metabolism.上皮性卵巢癌铂耐药：上皮-间充质转化的相互作用与重编程代谢相关联。
J Transl Med. 2022 Dec 3;20(1):556. doi: 10.1186/s12967-022-03776-y.
7
Identification of keygenes, miRNAs and miRNA-mRNA regulatory pathways for chemotherapy resistance in ovarian cancer.卵巢癌化疗耐药关键基因、miRNA及miRNA-mRNA调控通路的鉴定
PeerJ. 2021 Nov 8;9:e12353. doi: 10.7717/peerj.12353. eCollection 2021.
8
High expression of RASAL1, a hub gene in the progression of liver cancer, suggests a poor prognostis.RASAL1（一种在肝癌进展中起核心作用的基因）的高表达提示预后不良。
Am J Transl Res. 2022 Apr 15;14(4):2540-2549. eCollection 2022.
9
Identification of a five genes prognosis signature for triple-negative breast cancer using multi-omics methods and bioinformatics analysis.利用多组学方法和生物信息学分析鉴定三阴性乳腺癌的五个基因预后标志物。
Cancer Gene Ther. 2022 Nov;29(11):1578-1589. doi: 10.1038/s41417-022-00473-2. Epub 2022 Apr 26.
10
Four genes relevant to pathological grade and prognosis in ovarian cancer.与卵巢癌病理分级和预后相关的四个基因。
Cancer Biomark. 2020;29(2):169-178. doi: 10.3233/CBM-191162.

本文引用的文献

1
Hormone Replacement Therapy and Risks of Various Cancers in Postmenopausal Women with De Novo or a History of Endometriosis.激素替代疗法与初发或有子宫内膜异位症病史的绝经后女性患各种癌症的风险
Cancers (Basel). 2024 Feb 16;16(4):809. doi: 10.3390/cancers16040809.
2
New Achievements from Molecular Biology and Treatment Options for Refractory/Relapsed Ovarian Cancer-A Systematic Review.难治性/复发性卵巢癌的分子生物学新进展及治疗选择——一项系统综述
Cancers (Basel). 2023 Nov 10;15(22):5356. doi: 10.3390/cancers15225356.
3
Unveiling the Significance of FGF8 Overexpression in Orchestrating the Progression of Ovarian Cancer.
揭示 FGF8 过表达在调控卵巢癌进展中的意义。
Int J Mol Sci. 2023 Sep 18;24(18):14239. doi: 10.3390/ijms241814239.
4
Investigating the role of Kinesin family in lung adenocarcinoma via integrated bioinformatics approach.通过综合生物信息学方法研究驱动蛋白家族在肺腺癌中的作用。
Sci Rep. 2023 Jun 17;13(1):9859. doi: 10.1038/s41598-023-36842-6.
5
The STRING database in 2023: protein-protein association networks and functional enrichment analyses for any sequenced genome of interest.2023 年的 STRING 数据库：针对任何感兴趣的测序基因组的蛋白质-蛋白质关联网络和功能富集分析。
Nucleic Acids Res. 2023 Jan 6;51(D1):D638-D646. doi: 10.1093/nar/gkac1000.
6
Proteomic changes in human spermatozoa during in vitro capacitation and acrosome reaction in normozoospermia and asthenozoospermia.正常精子症和弱精子症患者精子在体外获能和顶体反应过程中的蛋白质组变化
Andrology. 2023 Jan;11(1):73-85. doi: 10.1111/andr.13289. Epub 2022 Sep 13.
7
Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis.整合转录组学和调控网络分析揭示了 let-7b-5p、SPIB 和 HLA-DPB1 在脓毒症中的作用。
Sci Rep. 2022 Jul 13;12(1):11963. doi: 10.1038/s41598-022-16183-6.
8
The diagnostic and prognostic implications of PRKRA expression in HBV-related hepatocellular carcinoma.PRKRA表达在乙型肝炎病毒相关肝细胞癌中的诊断和预后意义
Infect Agent Cancer. 2022 Jun 21;17(1):34. doi: 10.1186/s13027-022-00430-6.
9
Multiple biomarkers are more accurate than a combination of carbohydrate antigen 125 and human epididymis protein 4 for ovarian cancer screening.对于卵巢癌筛查，多种生物标志物比糖类抗原125和人附睾蛋白4联合检测更准确。
Obstet Gynecol Sci. 2022 Jul;65(4):346-354. doi: 10.5468/ogs.22017. Epub 2022 Apr 21.
10
DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update).DAVID：一个用于基因列表功能富集分析和功能注释的网络服务器（2021 更新）。
Nucleic Acids Res. 2022 Jul 5;50(W1):W216-W221. doi: 10.1093/nar/gkac194.