Chukwuma Ifeoma F, Okafor Kennedy Chinedu, Apeh Victor O, Nworah Florence N, Odo Chigozie Paul, Okafor Ijeoma Peace, Anoh Kelvin, Anthony Okoronkwo Chukwunenye
Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Enugu State, 410001, Nigeria.
Department of Genetics and Biotechnology, Faculty of Biological Sciences, University of Nigeria, Enugu State, 410001, Nigeria.
Heliyon. 2024 Aug 22;10(18):e36586. doi: 10.1016/j.heliyon.2024.e36586. eCollection 2024 Sep 30.
The quest for novel antioxidant and anti-inflammatory medications from medicinal plants is crucial since the plants contain bioactive compounds with a better efficacy and safety profile than orthodox therapy. This study harnesses the capabilities of mechatronics-driven Agilent Gas Chromatography, deploying , and in silico models to unravel the antioxidant and anti-inflammatory attributes within ethanol extract (CPEE). Employing gas chromatography-mass spectroscopy (GC-MS), our analysis efficiently segregates and evaluates volatile compound mixtures, a technique renowned for identifying organic compounds, as exemplified by its success in detecting fatty acids in food and resin acids in water. Using gas chromatography-mass spectrometry (GC-MS) and GC-FID analyses, this paper ascertains the comprehensive phytochemical composition of CPEE. Also, Molecular interactions of identified compounds with cyclooxygenase (COX-2) implicated in inflammatory urpsurge is verified. GC-MS and GC-FID analyses unveil 41 phytoconstituents within CPEE. Based on the research, CPEE demonstrated potential in inhibiting thiobarbituric acid-reactive substances, nitric oxide, and phospholipase lipase A2 with inhibition rates of 2.284, 6.547, and 66.8 μg/mL respectively. experiments confirm CPEE's efficacy in inhibiting granuloma tissue formation, lipid peroxidation, and neutrophil counts compared to untreated rats. Moreover, CPEE elicited a significant (P < 0.05) increase in the activities of SOD, CAT, and GSH concentrations while decreasing C-reactive protein, signifying promising therapeutic potential. Highlighting interactions between top-scoring phytoligands (epicatechin, catechin, and kaempferol) and COX-2, the findings underscore their drug-like characteristics, favorable pharmacokinetics, and enhanced safety toxicity profiles. Results from , and in silico studies, highlights CPEE remarkable antioxidant and anti-inflammatory potentials.
从药用植物中寻找新型抗氧化和抗炎药物至关重要，因为这些植物所含的生物活性化合物比传统疗法具有更好的疗效和安全性。本研究利用机电一体化驱动的安捷伦气相色谱仪、气相色谱-质谱联用仪以及计算机模拟模型，来揭示乙醇提取物（CPEE）中的抗氧化和抗炎特性。采用气相色谱-质谱联用技术（GC-MS），我们的分析能够有效地分离和评估挥发性化合物混合物，这是一种以鉴定有机化合物而闻名的技术，例如它成功地检测出了食物中的脂肪酸和水中的树脂酸。通过气相色谱-质谱联用（GC-MS）和气相色谱-火焰离子化检测（GC-FID）分析，本文确定了CPEE的全面植物化学成分。此外，还验证了所鉴定化合物与参与炎症爆发的环氧合酶（COX-2）的分子相互作用。GC-MS和GC-FID分析揭示了CPEE中含有41种植物成分。基于该研究，CPEE在抑制硫代巴比妥酸反应性物质、一氧化氮和磷脂酶A2方面表现出潜力，抑制率分别为2.284、6.547和66.8μg/mL。实验证实，与未处理的大鼠相比，CPEE在抑制肉芽肿组织形成、脂质过氧化和中性粒细胞计数方面具有功效。此外，CPEE能显著（P<0.05）提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活性以及谷胱甘肽（GSH）的浓度，同时降低C反应蛋白，显示出有前景的治疗潜力。研究突出了得分最高的植物配体（表儿茶素、儿茶素和山奈酚）与COX-2之间的相互作用，结果强调了它们类似药物的特性、良好的药代动力学以及增强的安全毒性特征。气相色谱-质谱联用仪、气相色谱-火焰离子化检测以及计算机模拟研究的结果突出了CPEE显著的抗氧化和抗炎潜力。
