Yılmaz Serhan, Blasco Tavares Pereira Lopes Filipa, Schlatzer Daniela, Ayati Marzieh, Chance Mark R, Koyutürk Mehmet
Department of Computer and Data Sciences, Case Western Reserve University, Cleveland, OH, 44106, United States.
Department of Nutrition, Case Western Reserve University, Cleveland, OH, 44106, United States.
Bioinform Adv. 2024 Jun 20;4(1):vbae077. doi: 10.1093/bioadv/vbae077. eCollection 2024.
We present RokaiXplorer, an intuitive web tool designed to address the scarcity of user-friendly solutions for proteomics and phospho-proteomics data analysis and visualization. RokaiXplorer streamlines data processing, analysis, and visualization through an interactive online interface, making it accessible to researchers without specialized training in proteomics or data science. With its comprehensive suite of modules, RokaiXplorer facilitates phospho-proteomic analysis at the level of phosphosites, proteins, kinases, biological processes, and pathways. The tool offers functionalities such as data normalization, statistical testing, activity inference, pathway enrichment, subgroup analysis, automated report generation, and multiple visualizations, including volcano plots, bar plots, heat maps, and network views. As a unique feature, RokaiXplorer allows researchers to effortlessly deploy their own data browsers, enabling interactive sharing of research data and findings. Overall, RokaiXplorer fills an important gap in phospho-proteomic data analysis by providing the ability to comprehensively analyze data at multiple levels within a single application.
Access RokaiXplorer at: http://explorer.rokai.io.
我们展示了RokaiXplorer，这是一个直观的网络工具，旨在解决蛋白质组学和磷酸化蛋白质组学数据分析及可视化方面用户友好型解决方案稀缺的问题。RokaiXplorer通过交互式在线界面简化了数据处理、分析和可视化过程，使没有蛋白质组学或数据科学专业培训的研究人员也能使用。凭借其全面的模块套件，RokaiXplorer有助于在磷酸化位点、蛋白质、激酶、生物过程和通路水平上进行磷酸化蛋白质组学分析。该工具提供数据归一化、统计测试、活性推断、通路富集、亚组分析、自动报告生成以及多种可视化功能，包括火山图、柱状图、热图和网络视图。作为一个独特的功能，RokaiXplorer允许研究人员轻松部署自己的数据浏览器，实现研究数据和结果的交互式共享。总体而言，RokaiXplorer通过在单个应用程序中提供在多个层面全面分析数据的能力，填补了磷酸化蛋白质组学数据分析中的一个重要空白。
可通过以下网址访问RokaiXplorer：http://explorer.rokai.io 。
