Wu Peishi, Wang Pingping, Cao Weiwei, Liu Ning, Zou Hang, Yang Guowei
State Key Laboratory of Optoelectronic Materials and Technologies, Nanotechnology Research Center, School of Materials Science & Engineering, Sun Yat-sen University, Guangzhou 510275, P. R. China.
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
ACS Omega. 2024 Sep 5;9(37):38846-38854. doi: 10.1021/acsomega.4c05003. eCollection 2024 Sep 17.
Matrine and oxymatrine are mainly obtained from using the high-temperature and prolonged solvent extraction methods currently employed in industries. In this study, an ultrafast and highly efficient method for extracting matrine and oxymatrine from at room temperature using laser technology, specifically, laser extraction, was demonstrated. The laser extraction rates for matrine and oxymatrine from at room temperature for 1 min were 266.40 and 936.80 mg(g·h), respectively. These rates were 1400 times higher than those achieved with conventional solvent extraction. These results mean that 1 min of laser extraction is equivalent to 24 h of solvent extraction. The reason for such a high efficiency is that laser-induced cavitation can accelerate the rapid release of alkaloid molecules in plant cells. Mass spectrum, nuclear magnetic resonance, and Fourier-transform infrared spectrum analyses of the extracted matrine and oxymatrine compounds confirmed that they are the same as the products of solvent extraction. Furthermore, it was found that the anticancer activity of laser-extracted compounds is slightly better than that of conventionally solvent-extracted ones, likely due to the slight change in the microstructure or conformation of these compounds under laser irradiation. These findings demonstrated that the laser extraction method was ultrafast and highly efficient, unveiling a novel approach to alkaloid extraction. This discovery will have significant implications for the extraction and utilization of alkaloids from plants.
苦参碱和氧化苦参碱主要通过目前工业上采用的高温长时间溶剂萃取法获得。在本研究中，展示了一种使用激光技术在室温下从[具体来源未提及]中提取苦参碱和氧化苦参碱的超快高效方法，即激光萃取。室温下1分钟从[具体来源未提及]中提取苦参碱和氧化苦参碱的激光萃取率分别为266.40和936.80毫克/（克·小时）。这些速率比传统溶剂萃取法高出1400倍。这意味着1分钟的激光萃取相当于24小时的溶剂萃取。如此高的效率的原因是激光诱导的空化作用可以加速植物细胞中生物碱分子的快速释放。对提取的苦参碱和氧化苦参碱化合物进行的质谱、核磁共振和傅里叶变换红外光谱分析证实，它们与溶剂萃取产物相同。此外，发现激光提取的化合物的抗癌活性略优于传统溶剂提取的化合物，这可能是由于这些化合物在激光照射下微观结构或构象发生了轻微变化。这些发现表明激光萃取方法超快且高效，揭示了一种提取生物碱的新方法。这一发现将对从植物中提取和利用生物碱具有重要意义。
