Grumelot Shaun, Ashkarran Ali Akbar, Jiwani Zahra, Ibrahim Rula, Mahmoudi Morteza
Department of Radiology and Precision Health Program, Michigan State University, East Lansing, Michigan 48824, United States.
ACS Omega. 2024 Aug 5;9(37):39188-39194. doi: 10.1021/acsomega.4c06166. eCollection 2024 Sep 17.
A colorimetric sensor array has been developed to differentiate various micro- and nanoplastic particles (MNPs), both pristine and those coated with a protein corona, in buffered water. This array utilizes five distinct cross-reactive chemo-responsive dyes, which exhibit changes in visible optical absorbance upon interaction with MNPs. Although no single dye responds exclusively to either pristine or protein-corona-coated MNPs, the collective shifts in color across all dyes create a unique molecular fingerprint for each type of MNP. This method demonstrates high sensitivity, capable of detecting MNPs of various sizes (50 nm, 100 nm, and 2 μm) and differentiating them from controls at concentrations as low as 10 ng/mL using standard chemometric techniques, ensuring accurate results without error. Additionally, the method can effectively distinguish between pristine and protein-corona-coated polystyrene MNPs. This colorimetric approach offers a rapid, cost-effective, and accurate method for monitoring MNP pollution and assessing their prior interactions with biological systems.
已开发出一种比色传感器阵列，用于区分缓冲水中的各种微塑料和纳米塑料颗粒（MNP），包括原始颗粒和被蛋白质冠层包覆的颗粒。该阵列利用五种不同的交叉反应化学响应染料，这些染料在与MNP相互作用时会在可见光吸收方面发生变化。虽然没有单一染料仅对原始的或被蛋白质冠层包覆的MNP作出反应，但所有染料颜色的集体变化为每种类型的MNP创造了独特的分子指纹。该方法显示出高灵敏度，能够检测各种尺寸（50纳米、100纳米和2微米）的MNP，并使用标准化学计量技术在低至10纳克/毫升的浓度下将它们与对照区分开来，确保结果准确无误。此外，该方法可以有效区分原始的和被蛋白质冠层包覆的聚苯乙烯MNP。这种比色方法为监测MNP污染以及评估它们与生物系统的先前相互作用提供了一种快速、经济高效且准确的方法。
