Zhang Jun, Luo Cheng, Long Haiying, Zhang Bin, Shan Hongtao, Hou Benli
Department of Hematology, the Second Affiliated Hospital of Guizhou Medical University, No. 3 Kangfu Road, Kaili City, Guizhou Province, 556000, China.
Int J Biol Markers. 2024 Dec;39(4):301-309. doi: 10.1177/03936155241283747. Epub 2024 Sep 23.
Multiple myeloma (MM) is a plasma cell malignancy characterized by abnormal plasma cell proliferation in the bone marrow. Circulating exosomal miRNA-451 is associated with the progression of many tumors, but the relationship between its expression and MM has not been reported. In this study, we aimed to investigate the clinical value of miRNA-451 as a biomarker for diagnosis and prognosis of multiple myeloma.
A total of 120 patients with multiple myeloma and 120 healthy control people were recruited in this study. The miRNA-451 expression in serum exosomes of participants was measured by quantitative real-time polymerase chain reaction, and the diagnostic value of miRNA-451 for multiple myeloma was assessed by receiver operating characteristic (ROC) curve. The correlation between miRNA-451 expression and plasma cells ratio and M protein content was analyzed by Pearson correlation coefficient. The prognosis of different miRNA-451 expression was evaluated by survival curves.
Results suggested that serum exosomal miRNA-451 expression was significantly decreased in patients with multiple myeloma rather than in the healthy controls. The ROC curve showed that area under the curve value of miRNA-451 was 0.888, suggesting that miRNA-451 had diagnostic value to multiple myeloma. Moreover, there was a negative correlation between miRNA-451 expression and plasma cells ratio or M protein content. Survival curves showed that patients with high miRNA-451 expression had a longer survival time, suggesting the value of miRNA-451 as a prognostic indicator of multiple myeloma.
We demonstrated the relationship between miRNA-451 expression and multiple myeloma, indicating that miRNA-451 in circulating exosomes may be an effective diagnostic biomarker and prognostic indicator for multiple myeloma.
多发性骨髓瘤（MM）是一种以骨髓中浆细胞异常增殖为特征的浆细胞恶性肿瘤。循环外泌体miRNA - 451与多种肿瘤的进展相关，但其表达与MM之间的关系尚未见报道。在本研究中，我们旨在探讨miRNA - 451作为多发性骨髓瘤诊断和预后生物标志物的临床价值。
本研究共纳入120例多发性骨髓瘤患者和120名健康对照者。采用定量实时聚合酶链反应检测参与者血清外泌体中miRNA - 451的表达，并通过受试者工作特征（ROC）曲线评估miRNA - 451对多发性骨髓瘤的诊断价值。采用Pearson相关系数分析miRNA - 451表达与浆细胞比例和M蛋白含量之间的相关性。通过生存曲线评估不同miRNA - 451表达水平的预后情况。
结果表明，多发性骨髓瘤患者血清外泌体miRNA - 451表达显著低于健康对照者。ROC曲线显示，miRNA - 451的曲线下面积值为0.888，表明miRNA - 451对多发性骨髓瘤具有诊断价值。此外，miRNA - 451表达与浆细胞比例或M蛋白含量呈负相关。生存曲线显示，miRNA - 451表达高的患者生存时间更长，提示miRNA - 451作为多发性骨髓瘤预后指标的价值。
我们证明了miRNA - 451表达与多发性骨髓瘤之间的关系，表明循环外泌体中的miRNA - 451可能是多发性骨髓瘤有效的诊断生物标志物和预后指标。
