Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Hematol Oncol. 2019 Oct;37(4):409-417. doi: 10.1002/hon.2639. Epub 2019 Jul 15.
Accumulating studies have focused on circulating microRNAs, which might be potential biomarkers for different malignancies. The aim of this study was to investigate the potential of serum exosomal microRNAs to be novel serum biomarkers for smouldering myeloma (SMM) or even multiple myeloma (MM). The levels of serum exosomal microRNAs and serum circulating microRNAs were measured in healthy individuals and patients with SMM (n = 20) or MM (n = 20). Serum exosomal microRNAs and serum circulating microRNAs were extracted from serum, and the expression levels of selected microRNAs were quantified by real-time polymerase chain reaction (PCR). The levels of serum exosome-derived miR-20a-5p, miR-103a-3p, and miR-4505 were significantly different among patients with MM, patients with SMM, and healthy individuals, while there were differences in the levels of let-7c-5p, miR-185-5p, and miR-4741 in patients with MM relative to those in SMM patients or healthy controls. Additionally, a significant correlation was rarely found between the levels of serum and exosomal microRNAs. This study shows that serum exosomal microRNAs can be used independently as novel serum biomarkers for MM.
越来越多的研究集中在循环 microRNAs 上，它们可能是不同恶性肿瘤的潜在生物标志物。本研究旨在探讨血清外泌体 microRNAs 是否可能成为冒烟型骨髓瘤 (SMM) 甚至多发性骨髓瘤 (MM) 的新型血清生物标志物。在健康个体和 SMM（n=20）或 MM（n=20）患者中测量了血清外泌体 microRNAs 和血清循环 microRNAs 的水平。从血清中提取血清外泌体 microRNAs 和血清循环 microRNAs，并通过实时聚合酶链反应 (PCR) 定量测定选定 microRNAs 的表达水平。在 MM 患者、SMM 患者和健康个体中，血清来源的 miR-20a-5p、miR-103a-3p 和 miR-4505 的水平存在显著差异，而在 MM 患者中，let-7c-5p、miR-185-5p 和 miR-4741 的水平与 SMM 患者或健康对照组存在差异。此外，血清和外泌体 microRNAs 之间很少发现显著相关性。本研究表明，血清外泌体 microRNAs 可作为 MM 的新型血清生物标志物独立使用。
