Ahmed Zulfiqar, Liu Min, Huang Shuntao, Xu Dequan
Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubie, People's Republic of China.
J Assist Reprod Genet. 2024 Nov;41(11):3105-3117. doi: 10.1007/s10815-024-03252-0. Epub 2024 Sep 23.
This study investigates the role of cation channel sperm associated 2 (CATSPER2) and sperm flagella 2 (SPEF2) genes in boar spermatogenesis, focusing on their association with sperm quality traits in boars.
Utilizing targeted next-generation sequencing, we identified and genotyped two polymorphisms in CATSPER2 (rs341636020G > A, rs326912346G > T) and three variants in SPEF2 (rs320839956A > G, rs334209514C > A, rs325319860C > T) across three boar breeds (Duroc, n = 181; Landrace, n = 87; Large White, n = 52).
Our results confirmed the presence of the specified single nucleotide polymorphisms (SNPs), adhering to association study criteria. In CATSPER2, significant associations were detected between rs341636020G > A and sperm curvilinear velocity (VCL) in Duroc and Landrace boars, and between rs326912346G > T and straight velocity (VSL) in Duroc and Large White boars. For SPEF2, rs320839956A > G was significantly linked to sperm viability in Duroc and Landrace and to sperm concentration (SCON) in Large White boars. Additionally, rs334209514C > A and rs325319860C > T showed significant associations with SCON and VCL respectively, in Doruc and Landrace boars.
Overall, our findings suggest that CATSPER2 and SPEF2 SNPs significantly impact boar sperm quality traits. These genetic markers have the potential to enhance boar fertility through selective breeding programs, contributing to the optimization of reproductive performance in pigs.
本研究调查阳离子通道精子相关蛋白2（CATSPER2）和精子鞭毛蛋白2（SPEF2）基因在公猪精子发生中的作用，重点关注它们与公猪精子质量性状的关联。
利用靶向二代测序技术，我们在三个公猪品种（杜洛克，n = 181；长白猪，n = 87；大白猪，n = 52）中鉴定并基因分型了CATSPER2基因的两个多态性位点（rs341636020G>A、rs326912346G>T）和SPEF2基因的三个变异位点（rs320839956A>G、rs334209514C>A、rs325319860C>T）。
我们的结果证实了特定单核苷酸多态性（SNP）的存在，符合关联研究标准。在CATSPER2基因中，杜洛克和长白公猪的rs341636020G>A与精子曲线速度（VCL）之间以及杜洛克和大白公猪的rs326912346G>T与直线速度（VSL）之间检测到显著关联。对于SPEF2基因，rs320839956A>G与杜洛克和长白猪的精子活力以及大白公猪的精子浓度（SCON）显著相关。此外，rs334209514C>A和rs325319860C>T分别与杜洛克和长白公猪的SCON和VCL显著相关。
总体而言，我们的研究结果表明，CATSPER2和SPEF2基因的SNP显著影响公猪精子质量性状。这些遗传标记有可能通过选择性育种计划提高公猪的繁殖力，有助于优化猪的繁殖性能。
