• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

岛脑区 GABA 能神经元促进小鼠异氟烷麻醉苏醒。

Zona Incerta GABAergic Neurons Facilitate Emergence from Isoflurane Anesthesia in Mice.

机构信息

Key Laboratory of Anesthesia and Organ Protestion of Ministry of Education (In Cultivation), Zunyi Medical University, Zunyi, China.

Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, China.

出版信息

Neurochem Res. 2024 Dec;49(12):3297-3307. doi: 10.1007/s11064-024-04230-9. Epub 2024 Sep 23.

DOI:10.1007/s11064-024-04230-9
PMID:39312079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11502554/
Abstract

The zona incerta (ZI) predominantly consists of gamma-aminobutyric acid (GABAergic) neurons, located adjacent to the lateral hypothalamus. GABA, acting on GABAA receptors, serves as a crucial neuromodulator in the initiation and maintenance of general anesthesia. In this study, we aimed to investigate the involvement of ZI GABAergic neurons in the general anesthesia process. Utilizing in-vivo calcium signal optical fiber recording, we observed a decrease in the activity of ZI GABAergic neurons during isoflurane anesthesia, followed by a significant increase during the recovery phase. Subsequently, we selectively ablated ZI GABAergic neurons to explore their role in general anesthesia, revealing no impact on the induction of isoflurane anesthesia but a prolonged recovery time, accompanied by a reduction in delta-band power in mice under isoflurane anesthesia. Finally, through optogenetic activation/inhibition of ZI GABAergic neurons during isoflurane anesthesia, we discovered that activation of these neurons facilitated emergence without affecting the induction process, while inhibition delayed emergence, leading to fluctuations in delta band activity. In summary, these findings highlight the involvement of ZI GABAergic neurons in modulating the emergence of isoflurane anesthesia.

摘要

中间脑区（ZI）主要由γ-氨基丁酸（GABAergic）神经元组成，位于外侧下丘脑附近。GABA 通过 GABAA 受体发挥作用，是全身麻醉开始和维持的关键神经调节剂。在这项研究中，我们旨在研究 ZI GABAergic 神经元在全身麻醉过程中的作用。我们利用在体钙信号光纤记录，观察到异氟烷麻醉期间 ZI GABAergic 神经元的活性降低，随后在恢复阶段显著增加。随后，我们选择性地消融 ZI GABAergic 神经元，以探索它们在全身麻醉中的作用，结果表明它们对异氟烷麻醉的诱导没有影响，但恢复时间延长，同时在异氟烷麻醉下的小鼠中 delta 波段功率降低。最后，通过在异氟烷麻醉期间对 ZI GABAergic 神经元进行光遗传激活/抑制，我们发现激活这些神经元有助于苏醒，而不影响诱导过程，而抑制则延迟苏醒，导致 delta 波段活动的波动。总之，这些发现强调了 ZI GABAergic 神经元在调节异氟烷麻醉苏醒中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/73c1067787bc/11064_2024_4230_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/a1cede0719cf/11064_2024_4230_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/9025339a173e/11064_2024_4230_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/e5dd388829e6/11064_2024_4230_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/73c1067787bc/11064_2024_4230_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/a1cede0719cf/11064_2024_4230_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/9025339a173e/11064_2024_4230_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/e5dd388829e6/11064_2024_4230_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd48/11502554/73c1067787bc/11064_2024_4230_Fig4_HTML.jpg

相似文献

1
Zona Incerta GABAergic Neurons Facilitate Emergence from Isoflurane Anesthesia in Mice.岛脑区 GABA 能神经元促进小鼠异氟烷麻醉苏醒。
Neurochem Res. 2024 Dec;49(12):3297-3307. doi: 10.1007/s11064-024-04230-9. Epub 2024 Sep 23.
2
[Activation of GABAergic neurons in the zona incerta accelerates anesthesia induction with sevoflurane and propofol without affecting anesthesia maintenance or awakening in mice].[激活未定带中的γ-氨基丁酸能神经元可加速小鼠使用七氟烷和丙泊酚诱导麻醉，且不影响麻醉维持或苏醒]
Nan Fang Yi Ke Da Xue Xue Bao. 2023 May 20;43(5):718-726. doi: 10.12122/j.issn.1673-4254.2023.05.06.
3
Ventral Tegmental Area Glutamatergic Neurons Facilitated Emergence From Isoflurane Anesthesia Involves Excitation of Lateral Septum GABA-ergic Neurons in Mice.腹侧被盖区谷氨酸能神经元促进异氟醚麻醉苏醒涉及小鼠外侧隔 GABA 能神经元的兴奋。
Anesth Analg. 2024 Aug 1;139(2):397-410. doi: 10.1213/ANE.0000000000006739. Epub 2023 Nov 27.
4
Zona incerta mediates early life isoflurane-induced fear memory deficits.不确定带中介了早期生活异氟醚诱发的恐惧记忆缺陷。
Sci Rep. 2024 Jul 2;14(1):15136. doi: 10.1038/s41598-024-66106-w.
5
Optogenetic/Chemogenetic Activation of GABAergic Neurons in the Ventral Tegmental Area Facilitates General Anesthesia via Projections to the Lateral Hypothalamus in Mice.光遗传学/化学遗传学激活腹侧被盖区的 GABA 能神经元通过投射到小鼠外侧下丘脑促进全身麻醉。
Front Neural Circuits. 2019 Nov 19;13:73. doi: 10.3389/fncir.2019.00073. eCollection 2019.
6
GABAergic neurons of anterior thalamic reticular nucleus regulate states of consciousness in propofol- and isoflurane-mediated general anesthesia.前丘脑网状核的 GABA 能神经元调节丙泊酚和异氟醚介导的全身麻醉意识状态。
CNS Neurosci Ther. 2024 Jun;30(6):e14782. doi: 10.1111/cns.14782.
7
Facilitation of Behavioral and Cortical Emergence from Isoflurane Anesthesia by GABAergic Neurons in Basal Forebrain.基底前脑 GABA 能神经元促进异氟烷麻醉中的行为和皮质出现。
J Neurosci. 2023 Apr 19;43(16):2907-2920. doi: 10.1523/JNEUROSCI.0628-22.2023. Epub 2023 Mar 3.
8
Activity of GABA neurons in the zona incerta and ventral lateral periaqueductal grey is biased towards sleep.在间脑区带和腹外侧导水管周围灰质中的 GABA 神经元的活动偏向于睡眠。
Sleep. 2023 Mar 9;46(3). doi: 10.1093/sleep/zsac306.
9
Effect of basal forebrain somatostatin and parvalbumin neurons in propofol and isoflurane anesthesia.基底前脑生长抑素和钙结合蛋白阳性神经元在丙泊酚和异氟醚麻醉中的作用。
CNS Neurosci Ther. 2021 Jul;27(7):792-804. doi: 10.1111/cns.13635. Epub 2021 Mar 25.
10
Suppressed GABAergic signaling in the zona incerta causes neuropathic pain in a thoracic hemisection spinal cord injury rat model.未定带中γ-氨基丁酸能信号传导受抑制会在胸段半横断脊髓损伤大鼠模型中引发神经性疼痛。
Neurosci Lett. 2016 Oct 6;632:55-61. doi: 10.1016/j.neulet.2016.08.035. Epub 2016 Aug 22.

本文引用的文献

1
The inescapable drive to sleep: Overlapping mechanisms of sleep and sedation.不可避免的睡眠驱动力：睡眠和镇静的重叠机制。
Science. 2021 Oct 29;374(6567):556-559. doi: 10.1126/science.abi8372. Epub 2021 Oct 28.
2
Norepinephrine modulates wakefulness via α1 adrenoceptors in paraventricular thalamic nucleus.去甲肾上腺素通过丘脑室旁核中的α1肾上腺素能受体调节觉醒。
iScience. 2021 Aug 21;24(9):103015. doi: 10.1016/j.isci.2021.103015. eCollection 2021 Sep 24.
3
GABAergic Neurons in the Dorsal-Intermediate Lateral Septum Regulate Sleep-Wakefulness and Anesthesia in Mice.
背侧隔核中间外侧部的 GABA 能神经元调控小鼠的睡眠-觉醒和麻醉。
Anesthesiology. 2021 Sep 1;135(3):463-481. doi: 10.1097/ALN.0000000000003868.
4
Dopaminergic Projections From the Ventral Tegmental Area to the Nucleus Accumbens Modulate Sevoflurane Anesthesia in Mice.从腹侧被盖区到伏隔核的多巴胺能投射调节小鼠的七氟烷麻醉。
Front Cell Neurosci. 2021 Apr 30;15:671473. doi: 10.3389/fncel.2021.671473. eCollection 2021.
5
Effect of basal forebrain somatostatin and parvalbumin neurons in propofol and isoflurane anesthesia.基底前脑生长抑素和钙结合蛋白阳性神经元在丙泊酚和异氟醚麻醉中的作用。
CNS Neurosci Ther. 2021 Jul;27(7):792-804. doi: 10.1111/cns.13635. Epub 2021 Mar 25.
6
Lateral Habenula Glutamatergic Neurons Modulate Isoflurane Anesthesia in Mice.外侧缰核谷氨酸能神经元调节小鼠异氟烷麻醉。
Front Mol Neurosci. 2021 Mar 4;14:628996. doi: 10.3389/fnmol.2021.628996. eCollection 2021.
7
Nucleus accumbens neurons expressing dopamine D1 receptors modulate states of consciousness in sevoflurane anesthesia.伏隔核多巴胺 D1 受体表达神经元调节七氟醚麻醉意识状态。
Curr Biol. 2021 May 10;31(9):1893-1902.e5. doi: 10.1016/j.cub.2021.02.011. Epub 2021 Mar 10.
8
Activation of GABAergic Neurons in the Rostromedial Tegmental Nucleus and Other Brainstem Regions Promotes Sedation and Facilitates Sevoflurane Anesthesia in Mice.中缝背核和其他脑干区域的 GABA 能神经元的激活可促进镇静并促进小鼠七氟醚麻醉。
Anesth Analg. 2021 Apr 1;132(4):e50-e55. doi: 10.1213/ANE.0000000000005387.
9
Dopamine neurons in the ventral periaqueductal gray modulate isoflurane anesthesia in rats.腹侧periaqueductal 灰色中的多巴胺神经元调节大鼠异氟醚麻醉。
CNS Neurosci Ther. 2020 Nov;26(11):1121-1133. doi: 10.1111/cns.13447. Epub 2020 Sep 2.
10
A common hub for sleep and motor control in the substantia nigra.黑质中睡眠和运动控制的共同枢纽。
Science. 2020 Jan 24;367(6476):440-445. doi: 10.1126/science.aaz0956.