Gui Huan, Liu Chengxi, He Haifeng, Zhang Jie, Chen Hong, Zhang Yi
Department of Anesthesiology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Guizhou Key Laboratory of Anesthesia and Organ Protection, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Front Cell Neurosci. 2021 Apr 30;15:671473. doi: 10.3389/fncel.2021.671473. eCollection 2021.
The role of the dopaminergic pathway in general anesthesia and its potential mechanisms are still unknown. In this study, we usedc-Fos staining combined with calcium fiber photometry recording to explore the activity of ventral tegmental area (VTA) dopaminergic neurons(VTA-DA) and nucleus accumbens (NAc) neurons during sevoflurane anesthesia. A genetically encoded dopamine (DA) sensor was used to investigate thefunction of the NAc in sevoflurane anesthesia. Chemogenetics and optogenetics were used to explore the role of the VTA-DA in sevofluraneanesthesia. Electroencephalogram (EEG) spectra, time of loss of righting reflex (LORR) and recovery of righting reflex (RORR) were recorded asassessment indicators. We found that VTA-DA and NAc neurons were inhibited during the induction period and were activated during the recoveryperiod of sevoflurane anesthesia. The fluorescence signals of dopamine decreased in the induction of and increased in the emergence from sevoflurane anesthesia.Activation of VTA-DA and the VTA-NAc pathway delayed the induction and facilitated the emergence accompanying with thereduction of delta band and the augmentation of the gamma band. These data demonstrate that VTA-DA neurons play a critical role in modulating sevofluraneanesthesia the VTA-NAc pathway.
多巴胺能通路在全身麻醉中的作用及其潜在机制仍不清楚。在本研究中，我们采用c-Fos染色结合钙纤维光度记录法，探究七氟醚麻醉期间腹侧被盖区（VTA）多巴胺能神经元（VTA-DA）和伏隔核（NAc）神经元的活性。使用一种基因编码的多巴胺（DA）传感器来研究NAc在七氟醚麻醉中的功能。采用化学遗传学和光遗传学方法探究VTA-DA在七氟醚麻醉中的作用。记录脑电图（EEG）频谱、翻正反射消失时间（LORR）和翻正反射恢复时间（RORR）作为评估指标。我们发现，在七氟醚麻醉诱导期VTA-DA和NAc神经元受到抑制，而在恢复期被激活。七氟醚麻醉诱导期多巴胺荧光信号降低，苏醒期升高。激活VTA-DA和VTA-NAc通路会延迟诱导并促进苏醒，同时伴有δ波带减少和γ波带增强。这些数据表明，VTA-DA神经元在通过VTA-NAc通路调节七氟醚麻醉中起关键作用。
