Greenlee J E, Sun M
Acta Neuropathol. 1985;67(3-4):226-9. doi: 10.1007/BF00687805.
Sera from patients with paraneoplastic cerebellar degeneration have been shown to contain high titers of antibody to human Purkinje cells. It is not known, however, whether these sera react with cerebellar material from species other than man. The present study was conducted to determine whether cerebellar tissue of nonhuman species could be used to screen human sera for anticerebellar antibodies and whether similarities in cerebellar antigens between nonhuman and human material might permit attempts to transmit cerebellar degeneration to experimental animals by passive transfer of patient sera. Sections of human, monkey, pig, sheep, cat, rabbit, and rat cerebellar tissue were overlaid with serial dilutions of positive sera from patients with cancer and with paraneoplastic cerebellar degeneration and were stained using indirect immunofluorescence methods. All sera stained specific Purkinje cells when reacted with monkey, pig, and rabbit cerebellar sections, but not all sera stained sheep, cat, or rat tissue. Immunofluorescent labeling of animal cerebellar tissue was less bright than that obtained with human cerebellar sections, and anticerebellar antibody titers were invariably lower when assayed with nonhuman than with human material. Although human anticerebellar antibodies react with cerebellar tissue from other animal species, patient-to-patient variation in staining is sufficiently great that not all patient sera might be suitable for passive transfer experiments, and that attempts to identify anticerebellar antibodies by reacting patient sera with nonhuman cerebellar tissue could be negative where these antibodies are in fact present and could be demonstrated using human material.
副肿瘤性小脑变性患者的血清已被证明含有高滴度的抗人浦肯野细胞抗体。然而，尚不清楚这些血清是否会与人类以外物种的小脑组织发生反应。本研究旨在确定非人类物种的小脑组织是否可用于筛查人类血清中的抗小脑抗体，以及非人类和人类组织之间小脑抗原的相似性是否允许通过被动转移患者血清将小脑变性传递给实验动物。将人、猴、猪、羊、猫、兔和大鼠小脑组织切片与癌症和副肿瘤性小脑变性患者的阳性血清系列稀释液覆盖，并使用间接免疫荧光法染色。所有血清与猴、猪和兔小脑切片反应时均能特异性地对浦肯野细胞染色，但并非所有血清都能对羊、猫或大鼠组织染色。动物小脑组织的免疫荧光标记不如人小脑切片明亮，用非人类材料检测时抗小脑抗体滴度始终低于用人材料检测时。虽然人类抗小脑抗体与其他动物物种的小脑组织发生反应，但患者之间染色的差异足够大，以至于并非所有患者血清都适合用于被动转移实验，并且用患者血清与非人类小脑组织反应来鉴定抗小脑抗体的尝试在实际上存在这些抗体并可以用人材料证明时可能为阴性。
