Western Guangdong Characteristic Biology and Medicine Engineering and Research Center, School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, PR China.
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, PR China.
Fitoterapia. 2024 Dec;179:106235. doi: 10.1016/j.fitote.2024.106235. Epub 2024 Sep 23.
Four new cuparene-type sesquiterpenes, flammuterpenols A - D (1-4), along with one known congener (5) were isolated from the solid culture of edible mushroom Flammulina filiformis. Their structures with a benzoxabicyclo[3.2.1]octane core were elucidated by integrated multiple spectroscopic techniques, electronic circular dichroism, and single crystal X-ray diffraction analysis. Biologically, compounds 1-5 were evaluated in vitro for their neuroprotective effects against 6-hydroxydopamine induced cell death in human neuroblastoma SH-SY5Y cells. All of them exhibited remarkable neuroprotective effects possessing the EC values ranging from 0.93 ± 0.02 to 10.28 ± 0.10 μM. These findings not only enrich the structural diversity of cuparene-type sesquiterpenes, but also provide potential candidates for the further development of the neuroprotective agents.
从可食用蘑菇金针菇 Flammulina filiformis 的固体培养物中分离得到了四个新的杯[4,5]-环辛二烯型倍半萜类化合物，flammuterpenols A-D（1-4），以及一个已知的同类物（5）。通过综合多种光谱技术、电子圆二色性和单晶 X 射线衍射分析，阐明了它们具有苯并[B]环辛烷核的结构。在体外，化合物 1-5 被评估了对人神经母细胞瘤 SH-SY5Y 细胞中 6-羟多巴胺诱导的细胞死亡的神经保护作用。它们均表现出显著的神经保护作用，EC 值范围为 0.93 ± 0.02 至 10.28 ± 0.10 μM。这些发现不仅丰富了杯[4,5]-环辛二烯型倍半萜类化合物的结构多样性，而且为进一步开发神经保护剂提供了潜在的候选物。
