Graduate School of Science, Technology and Innovation, Kobe University.
School of Agriculture, Meiji University.
Microbes Environ. 2024;39(3). doi: 10.1264/jsme2.ME24007.
Extracellular membrane vesicles (MVs) caused by the artificial production of polyhydroxybutyrate (PHB) were previously detected in Escherichia coli. We herein observed MV biogenesis in the mutant strain (-PHB) of the natural PHB producer, Cupriavidus necator H16. This inverse relationship was revealed through comparative electron microscopic ana-lyses of wild-type and mutant strains. Based on these results, we speculate that a physiological relationship exists between MV biogenesis and PHB biosynthesis. Therefore, we propose the potential of MV biogenesis as a fermentative "stress marker" for monitoring the performance of target polymer-producing microbial platforms.
先前在人工生产聚羟基丁酸酯（PHB）的大肠杆菌中检测到细胞外膜囊泡（MVs）。本研究在天然 PHB 生产者希瓦氏菌属（Cupriavidus necator）H16 的突变株（-PHB）中观察到 MV 的生物发生。通过对野生型和突变株的比较电子显微镜分析揭示了这种相反的关系。基于这些结果，我们推测 MV 的生物发生与 PHB 的生物合成之间存在生理关系。因此，我们提出了 MV 生物发生作为监测目标聚合物生产微生物平台性能的发酵“应激标志物”的潜力。
