Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA Cawangan Negeri Sembilan, Kampus Kuala Pilah, Kuala Pilah, Negeri Sembilan, Malaysia.
J Med Primatol. 2024 Oct;53(5):e12737. doi: 10.1111/jmp.12737.
The gut microbiota plays an important role in primates, which may be associated with their habitat. In Malaysia, pig-tailed macaques (Macaca nemestrina) live in different habitat environments and have traditionally been used for coconut plucking for more than a century. There is currently no information regarding the gut microbiota of this macaque in Malaysia. To address this oversight, this study employed a fecal metabarcoding approach to determine the gut microbiota composition of pig-tailed macaques and establish how these microbial communities correspond with the macaque external environments of residential area, forest edge, and fragmented forest.
To determine this connection, 300 paired-end sequences of 16S rRNA were amplified and sequenced using the MiSeq platform.
In the pig-tailed macaque fecal samples, we identified 17 phyla, 40 orders, 52 families, 101 genera, and 139 species of bacteria. The most prevalent bacterial families in the gut of pig-tailed macaques were Firmicutes (6.31%) and Proteobacteria (0.69%). Our analysis did not identify a significant difference between the type of environmental habitat and the gut microbiota composition of these macaques.
There was great variation in the population richness and bacterial community structure. The abundance of Firmicutes and Proteobacteria helps this macaque digest food more easily while maintaining a healthy gut microbiota diversity. Exploring the gut microbiota provides an initial effort to support pig-tailed macaque conservation in the future.
肠道微生物群在灵长类动物中起着重要作用，这可能与它们的栖息地有关。在马来西亚，猪尾猕猴（Macaca nemestrina）生活在不同的栖息地环境中，并且传统上被用于采摘椰子已有一个多世纪。目前，关于马来西亚这种猕猴的肠道微生物群的信息还没有。为了解决这一疏忽，本研究采用粪便代谢组学方法来确定猪尾猕猴的肠道微生物群组成，并确定这些微生物群落与居住区域、森林边缘和破碎森林等猕猴外部环境的对应关系。
为了确定这种联系，我们使用 MiSeq 平台扩增并测序了 300 对 16S rRNA 的末端序列。
在猪尾猕猴的粪便样本中，我们鉴定出了 17 个门、40 个目、52 个科、101 个属和 139 个种细菌。猪尾猕猴肠道中最常见的细菌科是厚壁菌门（Firmicutes）（6.31%）和变形菌门（Proteobacteria）（0.69%）。我们的分析没有发现环境栖息地类型和这些猕猴肠道微生物群组成之间存在显著差异。
种群丰富度和细菌群落结构存在很大差异。厚壁菌门和变形菌门的丰度有助于这种猕猴更轻松地消化食物，同时保持健康的肠道微生物群多样性。探索肠道微生物群为未来支持猪尾猕猴的保护提供了初步努力。
