大肠杆菌生长和分裂过程中细胞质膜的合成。呼吸性硝酸还原酶的分散行为。
Synthesis of cytoplasmic membrane during growth and division of Escherichia coli. Dispersive behaviour of respiratory nitrate reductase.
作者信息
Cadenas E, Garland P B
出版信息
Biochem J. 1979 Oct 15;184(1):45-50. doi: 10.1042/bj1840045.
Abstract
We have used the penicillin selection method of Autissier & Kepes [(1972) Biochimie 54, 93--101] to study the segregation of membrane-bound respiratory nitrate reductase (EC 1.9.6.1) in Escherichia coli for the three generations after cessation of nitrate reductase synthesis caused by withdrawal of nitrate from the growth medium. We also included a physical separation procedure that permitted direct assay for nitrate reductase activity among all fractions produced by the penicillin selection method. We conclude that the segregation of nitrate reductase after cell division is dispersive, and not semi-conservative as proposed by Autissier & Kepes (1972).
摘要
我们采用了奥蒂西耶与凯佩斯的青霉素选择法[（1972年）《生物化学》54卷，93 - 101页]，以研究在因从生长培养基中去除硝酸盐而导致硝酸盐还原酶合成停止后，大肠杆菌中膜结合呼吸硝酸盐还原酶（EC 1.9.6.1）在三代中的分离情况。我们还纳入了一种物理分离程序，该程序允许对青霉素选择法产生的所有组分中的硝酸盐还原酶活性进行直接测定。我们得出结论，细胞分裂后硝酸盐还原酶的分离是分散性的，而非如奥蒂西耶与凯佩斯（1972年）所提出的半保留性。
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