Department of Ophthalmology, Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, People's Republic of China; Shanghai Frontiers Science Research Base of Intelligent Optoelectronics and Perception, Institute of Optoelectronics, Fudan University, Shanghai, 200438, People's Republic of China; State Key Laboratory of Integrated Chips and Systems, Frontier Institute of Chip and System, Fudan University, Shanghai, 200438, People's Republic of China.
Department of Materials Science & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, 200438, People's Republic of China.
Biosens Bioelectron. 2025 Jan 1;267:116786. doi: 10.1016/j.bios.2024.116786. Epub 2024 Sep 18.
Technologies that established in vivo evaluations of soft-tissue biomechanics and temperature are essential to biological research and clinical diagnostics, particularly for a wide range of eye-related diseases such as glaucoma. Of importance are advanced bioelectronic devices for high-precise monitoring of intraocular pressure (IOP) and various ocular temperatures, as clinically proven uses for glaucoma diagnosis. Existing characterization methods are temporary, single point, and lack microscale resolution, failing to measure continuous IOP fluctuation across the long-term period. Here, this work presents a multi-functional smart contact lens, capable of rapidly capturing IOP fluctuation and ocular surface temperature (OST) for assistance for clinical use. The microscale device design is programmable and determined by finite element analysis simulation, with detailed experiments of ex vivo porcine eyeballs. Such compact bioelectronics can provide high-precise measurement with sensitivity of 0.03% mmHg and 1.2 Ω °C in the range of Δ2∼50 mmHg and 30-50 °C, respectively. In vivo tests of bio-integration with a living rabbit can evaluate real-time IOP fluctuation and OST, as of biocompatibility assessments verified through cellular and animal experiments. The resultant bioelectronic devices for continuous precise characterization of living eyeballs can offer broad utility for hospital diagnosis of a wide range of eye-related disorders.
用于评估软组织生物力学和温度的体内技术对于生物研究和临床诊断至关重要，特别是对于青光眼等广泛的眼部相关疾病。先进的生物电子设备对于高精度监测眼内压 (IOP) 和各种眼部温度非常重要，因为临床已经证明这些设备可用于青光眼诊断。现有的表征方法是暂时的、单点的，缺乏微观分辨率，无法测量长期内 IOP 的连续波动。在这里，这项工作提出了一种多功能智能隐形眼镜，能够快速捕捉 IOP 波动和眼部表面温度 (OST)，以协助临床使用。微尺度设备设计是可编程的，并通过有限元分析模拟确定，对离体猪眼球进行了详细的实验。这种紧凑型生物电子设备可以提供高精度测量，在 Δ2∼50 mmHg 和 30-50°C 的范围内，灵敏度分别为 0.03% mmHg 和 1.2 Ω°C。通过对活兔进行生物整合的体内测试，可以评估实时 IOP 波动和 OST，并且通过细胞和动物实验验证了其生物相容性评估。用于连续精确表征活体眼球的生物电子设备可为医院诊断广泛的眼部相关疾病提供广泛的应用。
