基于实时荧光定量PCR技术开发分子标记以检测市售苋属产品中的亚麻籽和芝麻籽。
Development of molecular markers based on real-time PCR to detect flax and sesame in commercial amaranth products.
作者信息
Kim Yeon Mi, Jang Cheol Seong
机构信息
Plant Genomics Laboratory, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon, 24341 Republic of Korea.
出版信息
Food Sci Biotechnol. 2024 May 13;33(14):3313-3322. doi: 10.1007/s10068-024-01584-2. eCollection 2024 Nov.
UNLABELLED
, , and are the most popular oilseed grains worldwide. Protein-rich contains bioactive peptides, is nutritious, and exhibits anti-allergic properties. is a primary trigger of anaphylaxis. also displays allergenic properties. A DNA marker assessable using quantitative real-time PCR was developed to detect and as allergenic contaminants of anti-allergenic . The efficiency of each primer set ranged from 90-98%, and high linear correlation (R > 0.99) was obtained between crossover values and the log DNA concentration. We established a Ct value of 0.1% of the binary as a cutoff. The practical application of the designed marker was confirmed by analyzing 20 commercial products. The qPCR system developed for detecting flaxseed and sesame can be applied for regulatory monitoring of allergenic substances in commercial amaranth-containing foods, thus contributing to protecting public health and safety.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-024-01584-2.
未标记
苋菜籽、亚麻籽和芝麻是全球最受欢迎的油籽谷物。富含蛋白质的苋菜籽含有生物活性肽，营养丰富，并具有抗过敏特性。芝麻是过敏反应的主要诱因。亚麻籽也具有致敏特性。开发了一种可通过定量实时聚合酶链反应评估的DNA标记，以检测作为抗敏苋菜籽致敏污染物的芝麻和亚麻籽。每个引物组的效率在90%至98%之间，交叉值与对数DNA浓度之间具有高度线性相关性（R＞0.99）。我们将二元混合物0.1%的Ct值确定为临界值。通过分析20种商业产品证实了所设计标记的实际应用。开发的用于检测亚麻籽和芝麻的定量聚合酶链反应系统可用于对含苋菜籽的商业食品中的致敏物质进行监管监测，从而有助于保护公众健康和安全。
补充信息
在线版本包含可在10.1007/s10068-024-01584-2获取的补充材料。
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