Lee Hana, Yang Seunghye, Lee Kyung Jin, Kim Si-Na, Jeong Ji-Seon, Kim Ki Young, Jung Cho-Rok, Jeon Sooyeon, Kwon Dayeon, Lee Sungin, Lee Hanbyeol, Park Chihye, Ahn Sun-Ju, Yoo Jongman, Son Mi-Young
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea.
Front Cell Dev Biol. 2024 Sep 12;12:1383893. doi: 10.3389/fcell.2024.1383893. eCollection 2024.
To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework to ensure consistency and reliability in the development, characterization, and application of intestinal organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, quality control measures, and functional assessments, aimed at fostering a standardized approach across diverse research initiatives. The implementation of these guidelines is anticipated to significantly contribute to the reproducibility and comparability of results in the burgeoning field of intestinal organoid research.
为加强肠道类器官的实际应用，制定标准化指南势在必行。本提议的标准化方案概述了一个全面框架，以确保肠道类器官的开发、表征和应用具有一致性和可靠性。推荐的指南涵盖关键参数，包括培养条件、关键质量属性、质量控制措施和功能评估，旨在促进不同研究项目采用标准化方法。预计这些指南的实施将极大地有助于肠道类器官研究这一新兴领域研究结果的可重复性和可比性。
