Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.
Shandong Engineering Laboratory of Sodium Hyaluronate and Its Derivatives, Shandong Focusfreda Biotech Co., Ltd., Qufu 273165, China.
Mar Drugs. 2024 Aug 31;22(9):399. doi: 10.3390/md22090399.
Hyaluronic acid (HA) is a well-known functional marine polysaccharide. The utilization and derivative development of HA are of great interest. Hyaluronan lyase has wide application prospects in the production of HA oligosaccharides and lower molecular weight HA. In this study, a strain of CGJ001 with high hyaluronan lyase activity was screened from industrial wastewater. This strain exhibited an impressive enzyme activity of 40,576 U/mL after being incubated for 14 h. Whole genome sequencing analysis revealed that CGJ001 contained a cluster of genes involved in HA degradation, transport, and metabolism. A newly identified enzyme responsible for glycosaminoglycan degradation was designated as HylEP0006. A strain of BL21(DE3)/ET-22b(+) was successfully constructed. HylEP0006 exhibited optimal degradation at 40 °C and pH 7.0, showing a high activity of 950,168.3 U/mg. HylEP0006 showed specific activity against HA. The minimum degradation fragment of HylEP0006 was hyaluronan tetrasaccharides, and HylEP0006 could efficiently degrade HA into unsaturated disaccharides (HA2), with HA2 as the final product. These characteristics indicate that HylEP0006 has a potential application prospect for the extraction and utilization of hyaluronic acid.
透明质酸（HA）是一种众所周知的功能海洋多糖。HA 的利用和衍生开发具有很大的兴趣。透明质酸裂解酶在 HA 低聚糖和低分子量 HA 的生产中具有广泛的应用前景。本研究从工业废水中筛选出一株具有高透明质酸裂解酶活性的菌株 CGJ001。该菌株经过 14 小时培养后，酶活达到 40576 U/mL。全基因组测序分析表明，CGJ001 含有参与 HA 降解、运输和代谢的基因簇。新鉴定的负责糖胺聚糖降解的酶被命名为 HylEP0006。成功构建了一株 BL21(DE3)/ET-22b(+)菌株。HylEP0006 在 40°C 和 pH7.0 下表现出最佳降解活性，酶活为 950168.3 U/mg。HylEP0006 对 HA 具有特异性活性。HylEP0006 的最小降解片段为透明质酸四糖，HylEP0006 可将 HA 高效降解为不饱和二糖（HA2），HA2 为最终产物。这些特性表明 HylEP0006 具有提取和利用透明质酸的潜在应用前景。
