Zhang Yue-Sheng, Gong Jin-Song, Yao Zhi-Yuan, Jiang Jia-Yu, Su Chang, Li Heng, Kang Chuan-Li, Liu Lei, Xu Zheng-Hong, Shi Jin-Song
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China.
Biotechnol Adv. 2022 Nov;60:108018. doi: 10.1016/j.biotechadv.2022.108018. Epub 2022 Jul 16.
It has long been found that hyaluronidases exist in a variety of organisms, playing their roles in various biological processes including infection, envenomation and metabolic regulation through degrading hyaluronan. However, exploiting them as a bioresource for specific applications had not been extensively studied until the latest decades. In recent years, new application scenarios have been developed, which extended the field of application, and emphasized the research value of hyaluronidase. This critical review comprehensively summarizes existing studies on hyaluronidase from different source, particularly in their structures, action patterns, and biological functions in human and mammals. Furthermore, we give in-depth insight into the resource mining and protein engineering process of hyaluronidase, as well as strategies for their high-level production, indicating that mixed strategies should be adopted to obtain well-performing hyaluronidase with efficiency. In addition, advances in application of hyaluronidase were summarized and discussed. Finally, prospects for future researches are proposed, highlighting the importance of further investigation into the characteristics of hyaluronidases, and the necessity of investigating their products for the development of their application value.
长期以来人们发现，透明质酸酶存在于多种生物体中，通过降解透明质酸在包括感染、中毒和代谢调节等各种生物过程中发挥作用。然而，直到最近几十年，才开始广泛研究将其开发为特定应用的生物资源。近年来，已经开发出了新的应用场景，这扩展了应用领域，并凸显了透明质酸酶的研究价值。这篇综述全面总结了来自不同来源的透明质酸酶的现有研究，特别是它们在人类和哺乳动物中的结构、作用模式和生物学功能。此外，我们深入探讨了透明质酸酶的资源挖掘和蛋白质工程过程，以及它们的高效生产策略，表明应采用混合策略来高效获得性能良好的透明质酸酶。此外，还总结并讨论了透明质酸酶应用方面的进展。最后，提出了未来研究的展望，强调了进一步研究透明质酸酶特性的重要性，以及研究其产物以开发其应用价值的必要性。
