Pineda Santiago, Martínez Garro Juliana María, Salazar Flórez Jorge Emilio, Agudelo-Pérez Sergio, Monroy Fernando P, Peláez Sánchez Ronald Guillermo
CES Biology, Science and Biotechnology School, CES University, Medellin 050021, Colombia.
Medicine Program, GEINCRO Research Group, School of Health Sciences, San Martín University Foundation, Sabaneta 055450, Colombia.
Trop Med Infect Dis. 2024 Sep 6;9(9):203. doi: 10.3390/tropicalmed9090203.
Leptospirosis is a disease caused by the bacteria of the genus, which can usually be acquired by humans through contact with urine from infected animals; it is also possible for this urine to contaminate soils and bodies of water. The disease can have deadly consequences in some extreme cases. Fortunately, until now, patients with leptospirosis have responded adequately to treatment with doxycycline and azithromycin, and no cases of antibiotic resistance have been reported. However, with the extensive use of such medications, more bacteria, such as and are becoming resistant. The purpose of this study is to determine the presence of genes related to antibiotic resistance in the genus using bioinformatic tools, which have not been undertaken in the past. Whole genomes from the 69 described species were downloaded from NCBI's GeneBank and analyzed using CARD (The Comprehensive Antibiotic Resistant Database) and RAST (Rapid Annotations using Subsystem Technology). After a detailed genomic search, 12 genes associated with four mechanisms were found: resistance to beta-lactamases, vancomycin, aminoglycoside adenylyltransferases, as well as multiple drug efflux pumps. Some of these genes are highly polymorphic among different species, and some of them are present in multiple copies in the same species. In conclusion, this study provides evidence of the presence of genes related to antibiotic resistance in the genomes of some species of the genus , and it is the starting point for future experimental evaluation to determine whether these genes are transcriptionally active in some species and serovars.
钩端螺旋体病是一种由钩端螺旋体属细菌引起的疾病，通常可通过人类接触受感染动物的尿液而感染；这种尿液也有可能污染土壤和水体。在某些极端情况下，该疾病可能会产生致命后果。幸运的是，到目前为止，钩端螺旋体病患者对强力霉素和阿奇霉素治疗反应良好，且未报告有抗生素耐药病例。然而，随着此类药物的广泛使用，更多细菌，如……正变得具有耐药性。本研究的目的是使用生物信息学工具确定钩端螺旋体属中与抗生素耐药性相关的基因的存在情况，这在过去尚未开展。从NCBI的基因库下载了69种已描述的钩端螺旋体物种的全基因组，并使用CARD（综合抗生素耐药数据库）和RAST（利用子系统技术的快速注释）进行分析。经过详细的基因组搜索，发现了与四种机制相关的12个基因：对β-内酰胺酶、万古霉素、氨基糖苷腺苷酸转移酶的耐药性，以及多种药物外排泵。其中一些基因在不同物种间具有高度多态性，并且其中一些基因在同一物种中以多个拷贝存在。总之，本研究提供了钩端螺旋体属某些物种基因组中存在与抗生素耐药性相关基因的证据，并且它是未来实验评估的起点，以确定这些基因在某些物种和血清型中是否具有转录活性。
