Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6DX, U.K.
Waters Corporation, Stamford Avenue, Wilmslow SK9 4AX, U.K.
Anal Chem. 2022 Mar 15;94(10):4141-4145. doi: 10.1021/acs.analchem.1c05614. Epub 2022 Mar 2.
Mass spectrometry (MS) allows for automated analysis of complex samples at high resolution without the need for labeling/derivatization. Liquid atmospheric pressure matrix-assisted laser desorption/ionization (LAP-MALDI) enables rapid sample preparation and MS analysis using microtiter-plate formats and high-performing mass spectrometers. We present a step change in high-speed, large-scale MS sample analysis of peptides at 20 samples/s and an enzymatic assay at 40 samples/s, i.e., an order of magnitude faster than current MS platforms. LAP-MALDI requires only low amounts of sample volume (<2 μL), of which only a fraction (<1%) is typically consumed, and allows for multiplexing and high-speed MS/MS analysis, demonstrated at ∼10 samples/s. Its high ion signal stability and similarity to electrospray ionization enables CVs below 10% and the analysis of multiply charged peptide ions at these extreme speeds. LAP-MALDI MS fulfills the speed requirements for large-scale population diagnostics and compound screening with the potential of analyzing >1 million samples per day.
质谱分析（MS）允许在无需标记/衍生化的情况下，对复杂样本进行高分辨率的自动化分析。液相大气压基质辅助激光解吸/电离（LAP-MALDI）可使用微孔板格式和高性能质谱仪实现快速样品制备和 MS 分析。我们实现了一个飞跃，可在 20 个样本/秒的速度下进行肽的高速、大规模 MS 样本分析，在 40 个样本/秒的速度下进行酶分析，即比当前的 MS 平台快一个数量级。LAP-MALDI 仅需要少量的样本体积（<2 μL），其中只有一小部分（<1%）通常会被消耗，并且允许进行多重和高速 MS/MS 分析，在约 10 个样本/秒时得到了证明。它具有高的离子信号稳定性和类似于电喷雾电离的特性，可实现低于 10%的 CV 和在这些极端速度下分析多电荷肽离子。LAP-MALDI MS 满足了大规模人群诊断和化合物筛选的速度要求，具有每天分析超过 100 万个样本的潜力。
