Department of Obstetrics and Gynecology, Minhang Hospital, Fudan University, Shanghai, China.
Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai, China.
Skin Res Technol. 2024 Oct;30(10):e70074. doi: 10.1111/srt.70074.
To investigate the screening value of a small-molecule probe to assess the methylation of PAX1 in cervical cancer.
The diagnostic threshold of the grayscale values for cervical lesions was assessed by plotting the Receiver Operating Characteristic (ROC) curve of subjects. Grayscale values were significantly different among the four groups (p < 0.05). Compared with the LSIL and cervicitis groups, a considerably higher grayscale value was found in the CA and high-grade squamous intraepithelial lesion (HSIL) groups (both p < 0.05).
The differential ROC curves of the grayscale values showed that the diagnostic Area Under Curve of the probe for cervicitis and low-grade squamous intraepithelial lesion (LSIL) was 0.8724 (95% CI = 0.7762-0.9685, p < 0.0001), for cervicitis and CA was 1.0000 (p < 0.0001), for the LSIL and HSIL was 0.5484 (95% CI = 0.3826-0.7142, p = 0.5755), and for the LSIL and CA was 0.7724 (95% CI = 0.6016-0.9432, p = 0.0138).
The small molecular probe has certain application value in differentiating the type of cervical lesions and has better efficacy in distinguishing cervical inflammatory and precancerous lesions from carcinogenesis, but less efficacy in determining the type of precancerous lesions.
探讨小分子探针评估宫颈癌 PAX1 甲基化的筛查价值。
通过受试者的接收者操作特征（ROC）曲线来评估宫颈病变的灰度值诊断阈值。四个组之间的灰度值存在显著差异（p<0.05）。与 LSIL 和宫颈炎组相比，CA 和高级别鳞状上皮内病变（HSIL）组的灰度值明显更高（均 p<0.05）。
灰度值的差异 ROC 曲线表明，探针对宫颈炎和低级别鳞状上皮内病变（LSIL）的诊断曲线下面积为 0.8724（95%置信区间=0.7762-0.9685，p<0.0001），对宫颈炎和 CA 的诊断曲线下面积为 1.0000（p<0.0001），对 LSIL 和 HSIL 的诊断曲线下面积为 0.5484（95%置信区间=0.3826-0.7142，p=0.5755），对 LSIL 和 CA 的诊断曲线下面积为 0.7724（95%置信区间=0.6016-0.9432，p=0.0138）。
小分子探针在区分宫颈病变类型方面具有一定的应用价值，在鉴别宫颈炎症性和癌前病变与癌变方面具有更好的疗效，但在确定癌前病变类型方面的疗效较差。
