尿液馏分的甲基化分析用于最佳的 CIN3 和宫颈癌检测。
Methylation analysis in urine fractions for optimal CIN3 and cervical cancer detection.
机构信息
Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Pathology, Cancer Center Amsterdam, Amsterdam, the Netherlands; Antoni van Leeuwenhoek/Netherlands Cancer Institute, Department of Gynecologic Oncology, Centre of Gynecologic Oncology Amsterdam, Amsterdam, the Netherlands.
Antoni van Leeuwenhoek/Netherlands Cancer Institute, Department of Gynecologic Oncology, Centre of Gynecologic Oncology Amsterdam, Amsterdam, the Netherlands.
出版信息
Papillomavirus Res. 2020 Jun;9:100193. doi: 10.1016/j.pvr.2020.100193. Epub 2020 Mar 13.
INTRODUCTION
Urine sampling is an interesting solution for CIN3 and cervical cancer detection. Urine can be separated in different fractions: full void urine, urine sediment and urine supernatant. We aimed to determine which urine fraction is most competent for CIN3 and cervical cancer detection by methylation analysis.
METHODS
Urine samples (27 controls, 30 CIN3 and 17 cervical cancer) were processed into 3 fractions and tested for 5 methylation markers (ASCL1, GHSR, LHX8, SST, ZIC1). We determined Spearman correlation coefficients between fractions, compared methylation levels and calculated AUCs for CIN3 and cancer detection.
RESULTS
In general strong correlations (r > 0.60) were found between urine fractions. Methylation levels increased significantly with severity of underlying disease in all urine fractions. CIN3 and controls differed significantly for 2 markers in full void urine, 4 markers in urine sediment and 1 marker in urine supernatant, with AUCs of 0.55-0.79. Comparison of cancer to controls was highly significant for all markers in all fractions, yielding AUCs of 0.87-0.99.
CONCLUSION
Methylation analysis performs excellent in all urine fractions for cervical cancer detection. Our results indicate the potential of CIN3 detection by urinary methylation analysis, and demonstrate that urine sediment performs best to detect CIN3.
简介
尿液采样是一种用于检测 CIN3 和宫颈癌的有趣方法。尿液可以分离成不同的部分：全尿、尿沉渣和尿上清液。我们旨在通过甲基化分析确定哪个尿液部分最适合用于 CIN3 和宫颈癌检测。
方法
对 27 个对照组、30 个 CIN3 和 17 个宫颈癌的尿液样本进行处理，分为 3 个部分，测试 5 个甲基化标志物（ASCL1、GHSR、LHX8、SST、ZIC1）。我们确定了各部分之间的斯皮尔曼相关系数，比较了甲基化水平，并计算了 CIN3 和癌症检测的 AUC。
结果
一般来说，尿液部分之间存在很强的相关性（r > 0.60）。在所有尿液部分中，甲基化水平随着潜在疾病的严重程度而显著增加。CIN3 和对照组在全尿中有 2 个标志物、尿沉渣中有 4 个标志物、尿上清液中有 1 个标志物的甲基化水平存在显著差异，AUC 值为 0.55-0.79。所有标志物在所有部分的癌症与对照组之间的比较均具有高度显著性，AUC 值为 0.87-0.99。
结论
甲基化分析在所有尿液部分中均能出色地用于宫颈癌检测。我们的结果表明，通过尿液甲基化分析检测 CIN3 具有潜力，并表明尿沉渣在检测 CIN3 方面表现最佳。
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