Zaninelli Tiago H, Saraiva-Santos Telma, Patlin Brielle H, Stratton Michael Slade, Chen David Y, Pinho-Ribeiro Felipe A
Division of Dermatology, Department of Medicine, Washington University School of Medicine in St. Louis, Saint Louis, MO, USA.
Division of Dermatology, Department of Medicine, Washington University School of Medicine in St. Louis, Saint Louis, MO, USA.
STAR Protoc. 2024 Dec 20;5(4):103345. doi: 10.1016/j.xpro.2024.103345. Epub 2024 Sep 26.
Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection. We then detail procedures for sample cross-section and confocal imaging.
神经周围浸润（PNI）是癌症复发和转移的一个重要风险因素；然而，其与癌症侵袭性相关的机制仍知之甚少。在此，我们展示了一种使用嗜神经性黑色素瘤细胞系在小鼠中建立PNI非手术模型的方案，该细胞系可从皮肤迁移至坐骨神经。我们描述了细胞培养与注射、肿瘤负荷测量、小鼠安乐死及组织解剖的步骤。然后，我们详细介绍了样本横断面和共聚焦成像的程序。
