Jiangxi Key Laboratory for Microscale Interdisciplinary Study, Institute for Advanced Study, College of Pharmacy, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
Jiangxi Key Laboratory for Microscale Interdisciplinary Study, Institute for Advanced Study, College of Pharmacy, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
Biochem Biophys Res Commun. 2024 Nov 12;733:150722. doi: 10.1016/j.bbrc.2024.150722. Epub 2024 Sep 19.
Cell-bound membrane vesicles (CBMVs), a novel type of membrane vesicles, have been identified through a series of characterization tools. However, the lipid composition of CBMVs has not yet been characterized. This study focuses on the differences in lipid composition between CBMVs and cell membranes. In order to determine the lipid composition of CBMVs and cell membranes of Human umbilical vein endothelial cells (HUVECs) and find out differential metabolites, this study was carried out by isolating CBMVs lipids and characterizing them using high-performance liquid chromatography tandem secondary mass spectrometry (LC-MS/MS). The results showed the presence of 213 up-regulated and 726 down-regulated lipids in CBMVs compared to cell membranes which produced CBMVs. There are lipids expressed in CBMVs and not in cell membranes: DGDG 18:0_8:0, DGDG O-8:0_16:1, DGDG O-26:7_26:7, DGDG O-16:3_26:7, TG 15:4_21:5_22:5; 4O, PC 49:11, PG 19:5_38:10, PI 60:14, PI 44:9, PI 25:2, PI 43:5, PI 50:10, PS 55:10. DGDG (digalactosyl diglyceride), MGDG (monogalactosyl diglyceride) belongs to galactosyl diglyceride, promotes fat catabolism, which also has antioxidant and anti-inflammatory effects, and unsaturated diacylglycerols are a class of antioxidant compounds, which enables CBMVs to have a therapeutic potential.
细胞结合膜囊泡（CBMVs），一种新型的膜囊泡，已经通过一系列的特征化工具被识别出来。然而，CBMVs 的脂质组成尚未被表征。本研究专注于 CBMVs 和细胞膜之间脂质组成的差异。为了确定人脐静脉内皮细胞（HUVECs）的 CBMVs 和细胞膜的脂质组成，并找出差异代谢物，本研究通过分离 CBMVs 脂质并用高效液相色谱串联二次质谱（LC-MS/MS）对其进行特征化来进行。结果表明，与产生 CBMVs 的细胞膜相比，CBMVs 中有 213 种上调和 726 种下调的脂质。有脂质在 CBMVs 中表达而不在细胞膜中表达：DGDG 18:0_8:0、DGDG O-8:0_16:1、DGDG O-26:7_26:7、DGDG O-16:3_26:7、TG 15:4_21:5_22:5；4O、PC 49:11、PG 19:5_38:10、PI 60:14、PI 44:9、PI 25:2、PI 43:5、PI 50:10、PS 55:10。DGDG（双半乳糖二甘油酯）、MGDG（单半乳糖二甘油酯）属于半乳糖二甘油酯，促进脂肪分解，还具有抗氧化和抗炎作用，不饱和二酰基甘油是一类抗氧化化合物，使 CBMVs 具有治疗潜力。
