Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos, Instituto Tecnológico de Sonora, 5 de febrero, 818 sur, C.P. 85000 Ciudad Obregón, Sonora, Mexico.
Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos, Instituto Tecnológico de Sonora, 5 de febrero, 818 sur, C.P. 85000 Ciudad Obregón, Sonora, Mexico.
Food Chem. 2025 Jan 15;463(Pt 3):141389. doi: 10.1016/j.foodchem.2024.141389. Epub 2024 Sep 21.
Smilax genus possesses bioactive properties attributed to phenolic compounds, which may exhibit antioxidant effects and inhibit the advanced glycation end products (AGEs). However, identifying these phenolic compounds and AGEs has become increasingly relevant to understanding such activities. This study aimed to identify phenolic compounds in extracts of Smilax spp. and evaluate their antioxidant and AGEs inhibitory activities. To achieve this, the Smilax genus was identified via PCR, and phenolic compounds including chlorogenic acid, naringenin-6-C-glucoside, quercetin, quercetin-3-O-glucoside, and myricetin were identified using HPLC-MS/MS. Antioxidant activity was assessed by ferric reducing antioxidant power (FRAP), and radicals such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2'-azino-bis-[3-ethyl-benzothiazoline]-6-sulfonic acid (ABTS), while AGEs inhibition was evaluated using a model system formed by bovine serum albumin-glucose. The highest antioxidant activity was 3612.18 mM TE/g, and the inhibition of AGEs was 52.44 %. These results demonstrate that Smilax spp. can inhibit AGEs, neutralize free radicals, and reduce compounds associated with antioxidant capacity.
菝葜属具有生物活性的酚类化合物，具有抗氧化作用，并能抑制晚期糖基化终产物（AGEs）。然而，识别这些酚类化合物和 AGEs 对于理解这些活性变得越来越重要。本研究旨在鉴定菝葜属提取物中的酚类化合物，并评估其抗氧化和 AGEs 抑制活性。为此，通过 PCR 鉴定菝葜属，并用 HPLC-MS/MS 鉴定绿原酸、柚皮苷-6-C-葡萄糖苷、槲皮素、槲皮素-3-O-葡萄糖苷和杨梅素等酚类化合物。抗氧化活性通过铁还原抗氧化能力（FRAP）评估，自由基如 2,2-二苯基-1-苦基肼（DPPH）和 2,2'-联氮-双-[3-乙基-苯并噻唑啉-6-磺酸]（ABTS），而 AGEs 抑制通过牛血清白蛋白-葡萄糖形成的模型系统进行评估。最高的抗氧化活性为 3612.18 mM TE/g，AGEs 抑制率为 52.44%。这些结果表明，菝葜属可以抑制 AGEs，中和自由基，并减少与抗氧化能力相关的化合物。
