Fraga Otto T, Silva Lucas A C, Silva José Cleydson F, Bevitori Rosângela, Silva Fredy D A, Pereira Welison A, Reis Pedro A B, Fontes Elizabeth P B
Department of Biochemistry and Molecular Biology, BIOAGRO, National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, Viçosa, MG, 36571.000, Brazil.
Biotechnology Laboratory, Embrapa Rice and Beans, Rodovia GO-462, Km 12, Santo Antônio de Goiás, GO, 75375-000, Brazil.
Planta. 2024 Sep 27;260(5):108. doi: 10.1007/s00425-024-04538-4.
Despite modulating senescence and drought responses, the GmERD15-like subfamily members are differentially induced by multiple stresses and diverge partially in stress signaling functions. The PAM2 motif represents a binding site for poly (A)-binding proteins (PABPs), often associated with RNA metabolism regulation. The PAM2-containing protein ERD15 stands out as a critical regulator of diverse stress responses in plants. Despite the relevance of the PAM2 motif, a comprehensive analysis of the PAM2 superfamily and ERD15-like subfamily in the plant kingdom is lacking. Here, we provide an extensive in silico analysis of the PAM2 superfamily and the ERD15-like subfamily in soybean, using Arabidopsis and rice sequences as prototypes. The Glycine max ERD15-like subfamily members were clustered in pairs, likely originating from DNA-based gene duplication, as the paralogs display high sequence conservation, similar exon/intron genome organization, and are undergoing purifying selection. Complementation analyses of an aterd15 mutant demonstrated that the plant ERD15-like subfamily members are functionally redundant in response to drought, osmotic stress, and dark-induced senescence. Nevertheless, the soybean members displayed differential expression profiles, biochemical activity, and subcellular localization, consistent with functional diversification. The expression profiles of Glyma04G138600 under salicylic acid (SA) and abscisic acid (ABA) treatments differed oppositely from those of the other GmERD15-like genes. Abiotic stress-induced coexpression analysis with soybean PABPs showed that Glyma04G138600 was clustered separately from other GmERD15s. In contrast to the AtERD15 stress-induced nuclear redistribution, Glyma04G138600 and Glyma02G260800 localized to the cytoplasm, while Glyma03G131900 fractionated between the cytoplasm and nucleus under normal and stress conditions. These data collectively indicate that despite modulating senescence and drought responses, the GmERD15-like subfamily members are differentially induced by multiple stresses and may diverge partially in stress signaling functions.
尽管GmERD15-like亚家族成员能够调节衰老和干旱反应，但它们在多种胁迫下的诱导方式存在差异，并且在胁迫信号传导功能上也部分有所不同。PAM2基序是多聚（A）结合蛋白（PABP）的结合位点，通常与RNA代谢调控相关。含有PAM2的蛋白ERD15是植物多种胁迫反应的关键调节因子。尽管PAM2基序具有重要意义，但目前缺乏对植物界PAM2超家族和ERD15-like亚家族的全面分析。在此，我们以拟南芥和水稻序列为原型，对大豆中的PAM2超家族和ERD15-like亚家族进行了广泛的计算机分析。大豆Glycine max ERD15-like亚家族成员成对聚类，可能源于基于DNA的基因复制，因为这些旁系同源物显示出高度的序列保守性、相似的外显子/内含子基因组结构，并且正在经历纯化选择。对拟南芥aterd15突变体的互补分析表明，植物ERD15-like亚家族成员在应对干旱、渗透胁迫和黑暗诱导的衰老方面功能冗余。然而，大豆成员表现出不同的表达谱、生化活性和亚细胞定位，这与功能多样化一致。水杨酸（SA）和脱落酸（ABA）处理下Glyma04G138600的表达谱与其他GmERD15-like基因相反。与大豆PABP的非生物胁迫诱导共表达分析表明，Glyma04G138600与其他GmERD15s分别聚类。与AtERD15胁迫诱导的核重新分布不同，Glyma04G138600和Glyma02G260800定位于细胞质，而Glyma03G131900在正常和胁迫条件下在细胞质和细胞核之间分布。这些数据共同表明，尽管GmERD15-like亚家族成员能够调节衰老和干旱反应，但它们在多种胁迫下的诱导方式存在差异，并且在胁迫信号传导功能上可能部分有所不同。
